Phase microscopy of technical and biological samples through random phase modulation with a difuser

Percival Almoro, Giancarlo Pedrini, Phanindra Narayan Gundu, Wolfgang Osten, Steen Grüner Hanson

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method. (C) 2010 Optical Society of America
    Original languageEnglish
    JournalOptics Letters
    Volume35
    Issue number7
    Pages (from-to)1028-1030
    ISSN0146-9592
    DOIs
    Publication statusPublished - 2010

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