Phase microscopy of technical and biological samples through random phase modulation with a difuser

Percival Almoro, Giancarlo Pedrini, Phanindra Narayan Gundu, Wolfgang Osten, Steen Grüner Hanson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method. (C) 2010 Optical Society of America
Original languageEnglish
JournalOptics Letters
Volume35
Issue number7
Pages (from-to)1028-1030
ISSN0146-9592
DOIs
Publication statusPublished - 2010

Fingerprint Dive into the research topics of 'Phase microscopy of technical and biological samples through random phase modulation with a difuser'. Together they form a unique fingerprint.

Cite this