PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice

Pie Huda, Tina Binderup, Martin Cramer Pedersen, Søren Roi Midtgaard, Dennis Ringkjøbing Elema, Andreas Kjær, Mikael Jensen, Lise Arleth

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    Abstract

    64Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold.
    Original languageEnglish
    Article numbere0129310
    JournalP L o S One
    Volume10
    Issue number7
    Number of pages14
    ISSN1932-6203
    DOIs
    Publication statusPublished - 2015

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