PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice

Pie Huda, Tina Binderup, Martin Cramer Pedersen, Søren Roi Midtgaard, Dennis Ringkjøbing Elema, Andreas Kjær, Mikael Jensen, Lise Arleth

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Abstract

64Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold.
Original languageEnglish
Article numbere0129310
JournalP L o S One
Volume10
Issue number7
Number of pages14
ISSN1932-6203
DOIs
Publication statusPublished - 2015

Cite this

Huda, P., Binderup, T., Pedersen, M. C., Midtgaard, S. R., Elema, D. R., Kjær, A., ... Arleth, L. (2015). PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice. P L o S One, 10(7), [e0129310]. https://doi.org/10.1371/journal.pone.0129310
Huda, Pie ; Binderup, Tina ; Pedersen, Martin Cramer ; Midtgaard, Søren Roi ; Elema, Dennis Ringkjøbing ; Kjær, Andreas ; Jensen, Mikael ; Arleth, Lise. / PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice. In: P L o S One. 2015 ; Vol. 10, No. 7.
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abstract = "64Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold.",
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Huda, P, Binderup, T, Pedersen, MC, Midtgaard, SR, Elema, DR, Kjær, A, Jensen, M & Arleth, L 2015, 'PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice', P L o S One, vol. 10, no. 7, e0129310. https://doi.org/10.1371/journal.pone.0129310

PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice. / Huda, Pie; Binderup, Tina; Pedersen, Martin Cramer; Midtgaard, Søren Roi; Elema, Dennis Ringkjøbing; Kjær, Andreas; Jensen, Mikael; Arleth, Lise.

In: P L o S One, Vol. 10, No. 7, e0129310, 2015.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice

AU - Huda, Pie

AU - Binderup, Tina

AU - Pedersen, Martin Cramer

AU - Midtgaard, Søren Roi

AU - Elema, Dennis Ringkjøbing

AU - Kjær, Andreas

AU - Jensen, Mikael

AU - Arleth, Lise

PY - 2015

Y1 - 2015

N2 - 64Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold.

AB - 64Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold.

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Huda P, Binderup T, Pedersen MC, Midtgaard SR, Elema DR, Kjær A et al. PET/CT Based In Vivo Evaluation of 64Cu Labelled Nanodiscs in Tumor Bearing Mice. P L o S One. 2015;10(7). e0129310. https://doi.org/10.1371/journal.pone.0129310