Peptide Synthesis Method and Solid Support for Use in the Method

Rolf Henrik Berg (Inventor), Kristoffer Almdal (Inventor), Walther Batsberg Pedersen (Inventor), Arne Holm (Inventor), James P. Tam (Inventor), Robert Bruce Merrifield (Inventor)

    Research output: Patent

    252 Downloads (Pure)

    Abstract

    A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,000-1,200,000. Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene. The method is particularly well-suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion. Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation.
    Original languageEnglish
    Patent numberUS5373053
    Filing date13/12/1994
    CountryDenmark
    Publication statusPublished - 1994

    Bibliographical note

    Also published as: US5258454 (A)

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