PCR assay for the detection of Campylobacter in marinated and non-marinated poultry products

Marianne Katzav, Pauliina Isohanni, Marianne Lund, Marjaana Hakkinen, Ulrike Lyhs

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    Abstract

    During a period of 9 months, 194 marinated and non-marinated poultry products were collected from retail shops in a defined area in Western Finland and tested for Campylobacter spp. using a conventional enrichment culture and Polymerase Chain Reaction (PCR) method. For marinated poultry products, the study involved modification of a commercial DNA isolation method. Using either a conventional culture or PCR method, a total of 25 (12.9%) of all investigated samples were Campylobacter positive. In marinated poultry products, Campylobacter was detected at a prevalence of 21.1% and 9.5% in turkey and chicken products, respectively. In August, there was a peak with 28.9% positive Campylobacter samples. Campylobacter inoculation tests were carried out to test the detection limit of both methods. The PCR method used is faster than microbiological analyses. However, enrichment of the samples is necessary due to the low occurrence of Campylobacter spp. in retail Finnish poultry products.
    Original languageEnglish
    JournalFood Microbiology
    Volume25
    Issue number7
    Pages (from-to)908-914
    ISSN0740-0020
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Poultry products
    • Campylobacter
    • Retail
    • PCR

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