PCR as a diagnostic test method for deduction of H. somni on trans-tracheal aspirated bronchoalveolar fluid from clinically normal calves and calves with pneumonia

J. M. D. Enemark, Øystein Angen, J. Thomsen

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    The aim of the present field study was to evaluate the diagnostic value of a species specific PCR test against Histophilus somni (earlier Haemophilus somnus). Trans-tracheal aspirated bronchoalveolar fluid (BAL) from 56 clinically normal healthy calves and 36 calves that developed pneumonia were collected in 6 different herds during September and November 2002. All 92 aspirations were analysed for Bovine Respiratory Syncytial virus (BRSV), Parainfluenza-3 virus, Bovine Coronavirus by antigen ELISA. Bacteria were detected by cultivation and H. somni additionally also by PCR. The results showed that 63% of the clinically normal calves harboured pathogenic bacteria (mainly P. multocida, M. haemolytica and H. somni) in the lower respiratory tract, and in 60% of these cases bacteria were found either in pure culture or as the predominant flora, something that could be expected to cause clinical disease. Among diseased calves, 97% contained bacteria in the lower respiratory tract, all of them classified as pure culture or many pathogenic bacteria in mixed culture. BRSV was detected in 53% of the diseased calves. A comparison of H. somni specific PCR test to cultivation showed PCR to be most sensitive test. No significant relation between pneumonia and detection of H. somni by PCR could be demonstrated, whereas a clear significant correlation between pneumonia and detection of H. somni by cultivation was found.
    Original languageEnglish
    Title of host publicationProceedings of the WBC Congress, Québec, Canada , 2004
    Number of pages837
    Volume029
    PublisherIVIS
    Publication date2004
    Publication statusPublished - 2004
    Event23rd World Buiatric Conference - Quebec City, Canada
    Duration: 11 Jul 200416 Jul 2004
    Conference number: 23

    Conference

    Conference23rd World Buiatric Conference
    Number23
    CountryCanada
    CityQuebec City
    Period11/07/200416/07/2004

    Fingerprint Dive into the research topics of 'PCR as a diagnostic test method for deduction of H. somni on trans-tracheal aspirated bronchoalveolar fluid from clinically normal calves and calves with pneumonia'. Together they form a unique fingerprint.

    Cite this