TY - JOUR
T1 - Patulin and secondary metabolite production by marine-derived Penicillium strains
AU - Vansteelandt, Marieke
AU - Kerzaon, Isabelle
AU - Blanchet, Elodie
AU - Fossi Tankoua, Olivia
AU - Robiou Du Pont, Thibaut
AU - Joubert, Yolaine
AU - Monteau, Fabrice
AU - Le Bizec, Bruno
AU - Frisvad, Jens C.
AU - Pouchus, Yves François
AU - Grovel, Olivier
PY - 2012
Y1 - 2012
N2 - Genus Penicillium represents an important fungal group regarding to its mycotoxin production. Secondary metabolomes of eight marine-derived strains belonging to subgenera Furcatum and Penicillium were investigated using dereplication by liquid chromatography (LC)–Diode Array Detector (DAD)–mass spectrometry (MS)/MS. Each strain was grown on six different culture media to enhance the number of observable metabolites.Thirty-two secondary metabolites were detected in crude extracts with twenty first observations for studied species. Patulin, a major mycotoxin, was classically detected in extracts of Penicillium expansum, and was also isolated from Penicillium antarcticum cultures, whose secondary metabolome is still to be done. These detections constituted the first descriptions of patulin in marine strains of Penicillium, highlighting the risk for shellfish and their consumers due to the presence of these fungi in shellfish farming areas.Patulin induced acute neurotoxicity on Diptera larvae, indicating the interest of this bioassay as an additional tool for detection of this major mycotoxin in crude extracts.
AB - Genus Penicillium represents an important fungal group regarding to its mycotoxin production. Secondary metabolomes of eight marine-derived strains belonging to subgenera Furcatum and Penicillium were investigated using dereplication by liquid chromatography (LC)–Diode Array Detector (DAD)–mass spectrometry (MS)/MS. Each strain was grown on six different culture media to enhance the number of observable metabolites.Thirty-two secondary metabolites were detected in crude extracts with twenty first observations for studied species. Patulin, a major mycotoxin, was classically detected in extracts of Penicillium expansum, and was also isolated from Penicillium antarcticum cultures, whose secondary metabolome is still to be done. These detections constituted the first descriptions of patulin in marine strains of Penicillium, highlighting the risk for shellfish and their consumers due to the presence of these fungi in shellfish farming areas.Patulin induced acute neurotoxicity on Diptera larvae, indicating the interest of this bioassay as an additional tool for detection of this major mycotoxin in crude extracts.
KW - LC–DAD–MS/MS dereplication
KW - Marine-derived Penicillium
KW - Neuroactivity
KW - Patulin
KW - Secondary metabolites
KW - Subgenus Furcatum
U2 - 10.1016/j.funbio.2012.06.005
DO - 10.1016/j.funbio.2012.06.005
M3 - Journal article
C2 - 22954338
SN - 1878-6146
VL - 116
SP - 954
EP - 961
JO - Fungal Biology
JF - Fungal Biology
IS - 9
ER -