Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs

Tomasz Stadejek, Lars E Larsen, Katarzyna Podgórska, Anette Bøtner, Sara Botti, Izabella Dolka, Michał Fabisiak, Peter Mikael Helweg Heegaard, Charlotte Kristiane Hjulsager, Tomasz Huć, Lise Kirstine Kvisgaard, Rafał Sapierzyński, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Studies from Eastern European countries proved that porcine reproductive and respiratory syndrome virus Type 1 (PRRSV-1) harbours high genetic diversity and that genetically divergent subtypes 2-4 circulate in this area. In the present study, we compared the pathogenicity of two different PRRSV-1 subtype 2 strains and a strain representing PRRSV-1 subtype 1. Four groups of 8-week-old specific pathogen free pigs were either infected with subtype 2 strain ILI6, subtype 2 strain or BOR59, subtype 1 strain 18794, or mock inoculated. The most pronounced clinical signs were observed in pigs infected with BOR59. Pigs from both subtype 2 strain infected groups exhibited significantly elevated mean body temperatures on DPI 2 compared to the other two groups, the difference remaining significant up to DPI 13 for the BOR59 group, only. The pigs in the latter group also displayed significantly highest levels of early viremia together with the most rapid APP response. Overall, the results indicated that BOR59 strain can be considered a highly pathogenic strain, similarly to subtype 3 strains Lena and SU1-bel, while the virulence of the other subtype 2 strain ILI6 was intermediate between BOR59 and subtype 1 strain.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume209
    Pages (from-to)13-19
    ISSN0378-1135
    DOIs
    Publication statusPublished - 2017

    Cite this

    Stadejek, Tomasz ; Larsen, Lars E ; Podgórska, Katarzyna ; Bøtner, Anette ; Botti, Sara ; Dolka, Izabella ; Fabisiak, Michał ; Heegaard, Peter Mikael Helweg ; Hjulsager, Charlotte Kristiane ; Huć, Tomasz ; Kvisgaard, Lise Kirstine ; Sapierzyński, Rafał ; Nielsen, Jens. / Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs. In: Veterinary Microbiology. 2017 ; Vol. 209. pp. 13-19.
    @article{63a5003d023448eca941367065f2e231,
    title = "Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs",
    abstract = "Studies from Eastern European countries proved that porcine reproductive and respiratory syndrome virus Type 1 (PRRSV-1) harbours high genetic diversity and that genetically divergent subtypes 2-4 circulate in this area. In the present study, we compared the pathogenicity of two different PRRSV-1 subtype 2 strains and a strain representing PRRSV-1 subtype 1. Four groups of 8-week-old specific pathogen free pigs were either infected with subtype 2 strain ILI6, subtype 2 strain or BOR59, subtype 1 strain 18794, or mock inoculated. The most pronounced clinical signs were observed in pigs infected with BOR59. Pigs from both subtype 2 strain infected groups exhibited significantly elevated mean body temperatures on DPI 2 compared to the other two groups, the difference remaining significant up to DPI 13 for the BOR59 group, only. The pigs in the latter group also displayed significantly highest levels of early viremia together with the most rapid APP response. Overall, the results indicated that BOR59 strain can be considered a highly pathogenic strain, similarly to subtype 3 strains Lena and SU1-bel, while the virulence of the other subtype 2 strain ILI6 was intermediate between BOR59 and subtype 1 strain.",
    author = "Tomasz Stadejek and Larsen, {Lars E} and Katarzyna Podg{\'o}rska and Anette B{\o}tner and Sara Botti and Izabella Dolka and Michał Fabisiak and Heegaard, {Peter Mikael Helweg} and Hjulsager, {Charlotte Kristiane} and Tomasz Huć and Kvisgaard, {Lise Kirstine} and Rafał Sapierzyński and Jens Nielsen",
    year = "2017",
    doi = "10.1016/j.vetmic.2017.05.011",
    language = "English",
    volume = "209",
    pages = "13--19",
    journal = "Veterinary Microbiology",
    issn = "0378-1135",
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    Stadejek, T, Larsen, LE, Podgórska, K, Bøtner, A, Botti, S, Dolka, I, Fabisiak, M, Heegaard, PMH, Hjulsager, CK, Huć, T, Kvisgaard, LK, Sapierzyński, R & Nielsen, J 2017, 'Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs', Veterinary Microbiology, vol. 209, pp. 13-19. https://doi.org/10.1016/j.vetmic.2017.05.011

    Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs. / Stadejek, Tomasz; Larsen, Lars E; Podgórska, Katarzyna; Bøtner, Anette; Botti, Sara; Dolka, Izabella; Fabisiak, Michał; Heegaard, Peter Mikael Helweg; Hjulsager, Charlotte Kristiane; Huć, Tomasz; Kvisgaard, Lise Kirstine; Sapierzyński, Rafał; Nielsen, Jens.

    In: Veterinary Microbiology, Vol. 209, 2017, p. 13-19.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs

    AU - Stadejek, Tomasz

    AU - Larsen, Lars E

    AU - Podgórska, Katarzyna

    AU - Bøtner, Anette

    AU - Botti, Sara

    AU - Dolka, Izabella

    AU - Fabisiak, Michał

    AU - Heegaard, Peter Mikael Helweg

    AU - Hjulsager, Charlotte Kristiane

    AU - Huć, Tomasz

    AU - Kvisgaard, Lise Kirstine

    AU - Sapierzyński, Rafał

    AU - Nielsen, Jens

    PY - 2017

    Y1 - 2017

    N2 - Studies from Eastern European countries proved that porcine reproductive and respiratory syndrome virus Type 1 (PRRSV-1) harbours high genetic diversity and that genetically divergent subtypes 2-4 circulate in this area. In the present study, we compared the pathogenicity of two different PRRSV-1 subtype 2 strains and a strain representing PRRSV-1 subtype 1. Four groups of 8-week-old specific pathogen free pigs were either infected with subtype 2 strain ILI6, subtype 2 strain or BOR59, subtype 1 strain 18794, or mock inoculated. The most pronounced clinical signs were observed in pigs infected with BOR59. Pigs from both subtype 2 strain infected groups exhibited significantly elevated mean body temperatures on DPI 2 compared to the other two groups, the difference remaining significant up to DPI 13 for the BOR59 group, only. The pigs in the latter group also displayed significantly highest levels of early viremia together with the most rapid APP response. Overall, the results indicated that BOR59 strain can be considered a highly pathogenic strain, similarly to subtype 3 strains Lena and SU1-bel, while the virulence of the other subtype 2 strain ILI6 was intermediate between BOR59 and subtype 1 strain.

    AB - Studies from Eastern European countries proved that porcine reproductive and respiratory syndrome virus Type 1 (PRRSV-1) harbours high genetic diversity and that genetically divergent subtypes 2-4 circulate in this area. In the present study, we compared the pathogenicity of two different PRRSV-1 subtype 2 strains and a strain representing PRRSV-1 subtype 1. Four groups of 8-week-old specific pathogen free pigs were either infected with subtype 2 strain ILI6, subtype 2 strain or BOR59, subtype 1 strain 18794, or mock inoculated. The most pronounced clinical signs were observed in pigs infected with BOR59. Pigs from both subtype 2 strain infected groups exhibited significantly elevated mean body temperatures on DPI 2 compared to the other two groups, the difference remaining significant up to DPI 13 for the BOR59 group, only. The pigs in the latter group also displayed significantly highest levels of early viremia together with the most rapid APP response. Overall, the results indicated that BOR59 strain can be considered a highly pathogenic strain, similarly to subtype 3 strains Lena and SU1-bel, while the virulence of the other subtype 2 strain ILI6 was intermediate between BOR59 and subtype 1 strain.

    U2 - 10.1016/j.vetmic.2017.05.011

    DO - 10.1016/j.vetmic.2017.05.011

    M3 - Journal article

    VL - 209

    SP - 13

    EP - 19

    JO - Veterinary Microbiology

    JF - Veterinary Microbiology

    SN - 0378-1135

    ER -