Passive dosing of pyrethroid insecticides to Daphnia magna: Expressing excess toxicity by chemical activity

Stine Nørgaard Schmidt, Jay Gan, A. C. Kretschmann, Nina Cedergreen, Philipp Mayer

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

111 Downloads (Pure)


Pyrethroid insecticides are nerve poisons and used as active ingredients in pesticide mixtures available for household and agriculture. The compounds are hydrophobic, and their strong sorption to organic material may result in decreasing exposure levels during toxicity tests and consequent underestimation of pyrethroid toxicity. This poster addresses three questions regarding the acute toxicity of pyrethroids towards the aquatic invertebrate Daphnia magna: (1) Is pyrethroid toxicity generally underestimated in the literature due to insufficiently controlled exposure levels? (2) At which chemical activity do pyrethroids exert their toxicity, and how similar are the effective chemical activities (Ea50) for different pyrethroids? (3) How much more toxic are pyrethroids relative to baseline toxicity? Toxicity experiments were conducted using passive dosing: Polydimethyl siloxane (PDMS) silicone was loaded with ?-cypermethrin, esfenvalerate and bifenthrin, respectively, and then applied to control the exposure to D. magna for 48 h by equilibrium partitioning. In this way, the exposure was kept constant since various losses were efficiently buffered by re-partitioning from the polymer. Based on results from the conducted passive dosing experiments and literature data, the three questions will be addressed in the following manner: (1) The effective concentration resulting in 50% immobilisation (EC50) will be determined for each of the three pyrethroids and compared to literature values, (2) Effective chemical activities resulting in 50% immobilisation (Ea50) will be estimated from pyrethroid EC50 values via the correlation of sub-cooled liquid solubility (S L, [mmol/L], representing a=1) and octanol to water partitioning ratios (Kow), (3) The excess toxicity observed for pyrethroids will be evaluated by comparing Ea50 values for individual pyrethroids to the chemical activity needed to initiate baseline toxicity (a=0.01-0.1).
Original languageEnglish
Title of host publicationSETAC Europe 25th Annual Meeting : Abstract Book
Number of pages1
Place of PublicationBarcelona, Spain
Publication date2015
Publication statusPublished - 2015
EventSETAC Europe 25th Annual Meeting - Barcelona, Spain
Duration: 3 May 20157 May 2015
Conference number: 25


ConferenceSETAC Europe 25th Annual Meeting
Internet address


Dive into the research topics of 'Passive dosing of pyrethroid insecticides to Daphnia magna: Expressing excess toxicity by chemical activity'. Together they form a unique fingerprint.

Cite this