Parts Characterization for Tunable Protein Expression

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Flow-seq combines flexible genome engineering methods with flow cytometry-based cell sorting and deep DNA sequencing to enable comprehensive interrogation of genotype to phenotype relationships. One application is to study the effect of specific regulatory elements on protein expression. Constructing targeted genomic variation around genomically integrated fluorescent marker genes enables rapid elucidation of the contribution of specific sequence variants to protein expression. Such an approach can be used to characterize the impact of modifications to the Shine-Dalgarno sequence in Escherichia coli.
Original languageEnglish
Title of host publicationSynthetic Metabolic Pathways
Volume1671
Publication date2018
Pages3-14
ISBN (Print)978-1-4939-7294-4
ISBN (Electronic)978-1-4939-7295-1
DOIs
Publication statusPublished - 2018
SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Flow cytometry cell sorting
  • Flow-seq
  • Next-generation sequencing
  • Shine-Dalgarno sequence

Cite this

Klausen, M. S., & Sommer, M. O. A. (2018). Parts Characterization for Tunable Protein Expression. In Synthetic Metabolic Pathways (Vol. 1671, pp. 3-14). Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-7295-1_1
Klausen, Michael Schantz ; Sommer, Morten Otto Alexander. / Parts Characterization for Tunable Protein Expression. Synthetic Metabolic Pathways. Vol. 1671 2018. pp. 3-14 (Methods in Molecular Biology).
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Klausen, MS & Sommer, MOA 2018, Parts Characterization for Tunable Protein Expression. in Synthetic Metabolic Pathways. vol. 1671, Methods in Molecular Biology, pp. 3-14. https://doi.org/10.1007/978-1-4939-7295-1_1

Parts Characterization for Tunable Protein Expression. / Klausen, Michael Schantz; Sommer, Morten Otto Alexander.

Synthetic Metabolic Pathways. Vol. 1671 2018. p. 3-14 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

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Klausen MS, Sommer MOA. Parts Characterization for Tunable Protein Expression. In Synthetic Metabolic Pathways. Vol. 1671. 2018. p. 3-14. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7295-1_1