Flow-seq combines flexible genome engineering methods with flow cytometry-based cell sorting and deep DNA sequencing to enable comprehensive interrogation of genotype to phenotype relationships. One application is to study the effect of specific regulatory elements on protein expression. Constructing targeted genomic variation around genomically integrated fluorescent marker genes enables rapid elucidation of the contribution of specific sequence variants to protein expression. Such an approach can be used to characterize the impact of modifications to the Shine-Dalgarno sequence in Escherichia coli.
|Title of host publication||Synthetic Metabolic Pathways|
|Publication status||Published - 2018|
|Series||Methods in Molecular Biology|
- Flow cytometry cell sorting
- Next-generation sequencing
- Shine-Dalgarno sequence