Partial Separation of Individual Enzyme Activities

An acyl carrier protein (ACP) dependent fatty acid synthetase (fas) from barley chloroplast stroma was purified five-fold by ammonium sulphate precipitation and gel filtration on Sephacryl S-300. The β-ketoacyl-ACP reductase, β-ketoacyl-ACP synthetase, acetyl-CoA:ACP transacylase and malonyl-CoA:ACP transacylaseactivities were resolved on the Sephacryl S-300 column with apparent molecular weights of respectively 125, 92,82 and 41 kilodalton. The fas activity exhibited an apparent molecular weight of 87 kilodalton resulting from theoverlapping portions of the component activities. A fifth component of the active fas, ACP, was separatedcompletely from the other four individual enzyme activities by the ammonium sulphate precipitation. When thefas purified by gel filtration was applied to a Ma-trex Gel Blue B column, the component activities were separatedinto two groups. A bound fraction contained all the malonyl-CoA:ACP transacylase whereas the [β-ketoacylsynthetase activity was exclusively present in the non-bound fraction. Neither the bound nor the non-bound fraction showed any fas activity alone, but complete reconstitution of fas activity was obtained when both protein fractio~ were combined. The barley chloroplast fas is therefore not a multifunctional protein butconsists of at least five separable components. Characterization with respect to cofactor requirements was alsoperformed. Variation of certain cofactor concentrations markedly altered the pattern of fatty acid synthesis.