Parallel optical sorting of biological cells using the generalized phase contrast method

Lars Rindorf, Minqiang Bu, Jesper Glückstad

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

Optical forces are used to fixate biological cells with optical tweezers where numerous biological parameters and phenomena can be studied. Optical beams carry a small momentum which generates a weak optical force, but on a cellular level this force is strong enough to allow for manipulation of biological cells in microfluidic systems exclusively using light. We demonstrate an optical cell sorter that uses simultaneous manipulation by multiple laser beams using the Generalized Phase Contrast method (GPC). The basic principle in an optical sorter is that
the radiation force of the optical beam can push the biological cell from one microfluidic sheath flow to another. By incorporating a spatial light modulator the manipulation can be made parallel with multiple laser beams. We claim advantages over the serial optical sorters with only a single laser beam that has been demonstrated by others.
Original languageEnglish
Title of host publicationProceedings of SPIE : Microfluidics, BioMEMS, and Medical Microsystems XII
Number of pages9
Volume8976
PublisherSPIE - International Society for Optical Engineering
Publication date2014
DOIs
Publication statusPublished - 2014
EventMicrofluidics, BioMEMS, and Medical Microsystems XII - San Francisco, California, United States
Duration: 2 Feb 20144 Feb 2014

Conference

ConferenceMicrofluidics, BioMEMS, and Medical Microsystems XII
CountryUnited States
CitySan Francisco, California
Period02/02/201404/02/2014
SeriesProceedings of SPIE, the International Society for Optical Engineering
Volume8976
ISSN0277-786X

Keywords

  • Cell sorter
  • GPC
  • Fourier optics
  • Spatial light modulators
  • Optofluidics
  • Biophotonics

Cite this

Rindorf, L., Bu, M., & Glückstad, J. (2014). Parallel optical sorting of biological cells using the generalized phase contrast method. In Proceedings of SPIE: Microfluidics, BioMEMS, and Medical Microsystems XII (Vol. 8976). SPIE - International Society for Optical Engineering. Proceedings of SPIE, the International Society for Optical Engineering, Vol.. 8976 https://doi.org/10.1117/12.2035321