Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa

Fatima AlZahra'a Alatraktchi*, Jafar Safaa Noori, Georgi Plamenov Tanev, John Mortensen, Maria Dimaki, Helle Krogh Johansen, Jan Madsen, Søren Molin, Winnie Edith Svendsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

411 Downloads (Pure)

Abstract

Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.
Original languageEnglish
Article numbere0194157
JournalP L o S One
Volume13
Issue number3
Number of pages9
ISSN1932-6203
DOIs
Publication statusPublished - 2018

Cite this

@article{5f947b49853d443c9fdf590b04158b82,
title = "Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa",
abstract = "Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.",
author = "Alatraktchi, {Fatima AlZahra'a} and Noori, {Jafar Safaa} and Tanev, {Georgi Plamenov} and John Mortensen and Maria Dimaki and Johansen, {Helle Krogh} and Jan Madsen and S{\o}ren Molin and Svendsen, {Winnie Edith}",
year = "2018",
doi = "10.1371/journal.pone.0194157",
language = "English",
volume = "13",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa. / Alatraktchi, Fatima AlZahra'a; Noori, Jafar Safaa; Tanev, Georgi Plamenov; Mortensen, John; Dimaki, Maria; Johansen, Helle Krogh; Madsen, Jan; Molin, Søren; Svendsen, Winnie Edith.

In: P L o S One, Vol. 13, No. 3, e0194157, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa

AU - Alatraktchi, Fatima AlZahra'a

AU - Noori, Jafar Safaa

AU - Tanev, Georgi Plamenov

AU - Mortensen, John

AU - Dimaki, Maria

AU - Johansen, Helle Krogh

AU - Madsen, Jan

AU - Molin, Søren

AU - Svendsen, Winnie Edith

PY - 2018

Y1 - 2018

N2 - Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.

AB - Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.

U2 - 10.1371/journal.pone.0194157

DO - 10.1371/journal.pone.0194157

M3 - Journal article

VL - 13

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 3

M1 - e0194157

ER -