PanKB: An interactive microbial pangenome knowledgebase for research, biotechnological innovation, and knowledge mining

Binhuan Sun, Liubov Pashkova, Pascal Aldo Pieters, Archana Sanjay Harke, Omkar Satyavan Mohite, Alberto Santos, Daniel C. Zielinski, Bernhard O. Palsson, Patrick Victor Phaneuf

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Abstract

The exponential growth of microbial genome data presents unprecedented opportunities for unlocking the potential of microorganisms. The burgeoning field of pangenomics offers a framework for extracting insights from this big biological data. Recent advances in microbial pangenomic research have generated substantial data and literature, yielding valuable knowledge across diverse microbial species. PanKB (pankb.org), a knowledgebase designed for microbial pangenomics research and biotechnological applications, was built to capitalize on this wealth of information. PanKB currently includes 51 pangenomes from 8 industrially relevant microbial families, comprising 8402 genomes, over 500 000 genes and over 7M mutations. To describe this data, PanKB implements four main components: (1) Interactive pangenomic analytics to facilitate exploration, intuition, and potential discoveries; (2) Alleleomic analytics, a pangenomic-scale analysis of variants, providing insights into intraspecies sequence variation and potential mutations for applications; (3) A global search function enabling broad and deep investigations across pangenomes to power research and bioengineering workflows; (4) A bibliome of 833 open-access pangenomic papers and an interface with an LLM that can answer in-depth questions using its knowledge. PanKB empowers researchers and bioengineers to harness the potential of microbial pangenomics and serves as a valuable resource bridging the gap between pangenomic data and practical applications. [GRAPHICS] .
Original languageEnglish
JournalNucleic Acids Research
Volume53
Issue numberD1
Pages (from-to)D806–D818
ISSN0305-1048
DOIs
Publication statusPublished - 2025

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