TY - JOUR
T1 - Oxidised fish oil does not influence established markers of oxidative stress in healthy human subjects
T2 - a randomized controlled trial
AU - Ottestad, Inger
AU - Vogt, Gjermund
AU - Retterstøl, Kjetil
AU - Myhrstad, Mari C.
AU - Haugen, Jens-Erik
AU - Nilsson, Astrid
AU - Ravn-Haren, Gitte
AU - Nordvi, Berit
AU - Brønner, Kristi W.
AU - Andersen, Lene F.
AU - Holven, Kirsten B.
AU - Ulven, Stine M.
PY - 2012
Y1 - 2012
N2 - Intake of fish oil reduces the risk of CHD and CHD deaths. Marine n-3 fatty acids (FA) are susceptible to oxidation, but to our knowledge,
the health effects of intake of oxidised fish oil have not previously been investigated in human subjects. The aim of the present study was
to investigate markers of oxidative stress, lipid peroxidation and inflammation, and the level of plasma n-3 FA after intake of oxidised fish
oil. In a double-blinded randomised controlled study, healthy subjects (aged 18–50 years, n 54) were assigned into one of three groups
receiving capsules containing either 8 g/d of fish oil (1·6 g/d EPA þ DHA; n 17), 8 g/d of oxidised fish oil (1·6 g/d EPA þ DHA; n 18) or
8 g/d of high-oleic sunflower oil (n 19). Fasting blood and morning spot urine samples were collected at weeks 0, 3 and 7. No significant
changes between the different groups were observed with regard to urinary 8-iso-PGF2a; plasma levels of 4-hydroxy-2-hexenal,
4-hydroxy-2-nonenal and a-tocopherol; serum high sensitive C-reactive protein; or activity of antioxidant enzymes in erythrocytes. A significant
increase in plasma level of EPA þ DHA was observed in both fish oil groups, but no significant difference was observed between
the fish oil groups. No changes in a variety of in vivo markers of oxidative stress, lipid peroxidation or inflammation were observed after
daily intake of oxidised fish oil for 3 or 7 weeks, indicating that intake of oxidised fish oil may not have unfavourable short-term effects in
healthy human subjects.
AB - Intake of fish oil reduces the risk of CHD and CHD deaths. Marine n-3 fatty acids (FA) are susceptible to oxidation, but to our knowledge,
the health effects of intake of oxidised fish oil have not previously been investigated in human subjects. The aim of the present study was
to investigate markers of oxidative stress, lipid peroxidation and inflammation, and the level of plasma n-3 FA after intake of oxidised fish
oil. In a double-blinded randomised controlled study, healthy subjects (aged 18–50 years, n 54) were assigned into one of three groups
receiving capsules containing either 8 g/d of fish oil (1·6 g/d EPA þ DHA; n 17), 8 g/d of oxidised fish oil (1·6 g/d EPA þ DHA; n 18) or
8 g/d of high-oleic sunflower oil (n 19). Fasting blood and morning spot urine samples were collected at weeks 0, 3 and 7. No significant
changes between the different groups were observed with regard to urinary 8-iso-PGF2a; plasma levels of 4-hydroxy-2-hexenal,
4-hydroxy-2-nonenal and a-tocopherol; serum high sensitive C-reactive protein; or activity of antioxidant enzymes in erythrocytes. A significant
increase in plasma level of EPA þ DHA was observed in both fish oil groups, but no significant difference was observed between
the fish oil groups. No changes in a variety of in vivo markers of oxidative stress, lipid peroxidation or inflammation were observed after
daily intake of oxidised fish oil for 3 or 7 weeks, indicating that intake of oxidised fish oil may not have unfavourable short-term effects in
healthy human subjects.
U2 - 10.1017/S0007114511005484
DO - 10.1017/S0007114511005484
M3 - Journal article
C2 - 22136711
SN - 0007-1145
VL - 108
SP - 315
EP - 326
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 2
ER -