Overexpression of cyclooxygenase-2 in adipocytes reduces fat accumulation in inguinal white adipose tissue and hepatic steatosis in high-fat fed mice

Niels Banhos Danneskiold-Samsøe, Si Brask Sonne, Jeppe Madura Larsen, Ann Normann Hansen, Even Fjære, Marie Sophie Isidor, Sidsel Rübner Petersen, Jeanette Henningsen, Ilenia Severi, Loris Sartini, Yvonne Schober, Jacqueline Wolf, W Andreas Nockher, Christian Wolfrum, Saverio Cinti, Christian Sina, Jacob B Hansen, Lise Madsen, Susanne Brix*, Karsten Kristiansen

*Corresponding author for this work

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Cyclooxygenases are known as important regulators of metabolism and immune processes via conversion of C20 fatty acids into various regulatory lipid mediators, and cyclooxygenase activity has been implicated in browning of white adipose tissues. We generated transgenic (TG) C57BL/6 mice expressing the Ptgs2 gene encoding cyclooxygenase-2 (COX-2) in mature adipocytes. TG mice fed a high-fat diet displayed marginally lower weight gain with less hepatic steatosis and a slight improvement in insulin sensitivity, but no difference in glucose tolerance. Compared to littermate wildtype mice, TG mice selectively reduced inguinal white adipose tissue (iWAT) mass and fat cell size, whereas the epididymal (eWAT) fat depot remained unchanged. The changes in iWAT were accompanied by increased levels of specific COX-derived lipid mediators and increased mRNA levels of interleukin-33, interleukin-4 and arginase-1, but not increased expression of uncoupling protein 1 or increased energy expenditure. Epididymal WAT (eWAT) in TG mice exhibited few changes except from increased infiltration with eosinophils. Our findings suggest a role for COX-2-derived lipid mediators from adipocytes in mediating type 2 immunity cues in subcutaneous WAT associated with decreased hepatic steatosis, but with no accompanying induction of browning and increased energy expenditure.
Original languageEnglish
Article number8979
JournalScientific Reports
Issue number1
Number of pages13
Publication statusPublished - 2019

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