Outcomes of in vitro fertilization with frozen-thawed sperm: An analysis of post-thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Vahid Zadmajid*, Elham Falahipour, Edris Ghaderi, Sune Riis Sørensen, Ian Anthony Ernest Butts

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are sired with fresh and frozen-thawed sperm. Cryopreserved sperm did not affect embryogenesis at 2- and 4-cell stages, but impaired embryonic development at 8-cell stage. Embryonic viability decreased at organogenesis, where only 34-49% of embryos showed viability with frozen-thawed sperm. Hatching success and percentage of normal hatched embryos declined when fertilized with frozen-thawed sperm. Considering only frozen-thawed cells the DMSO-5%, METH-5%, and METH-10% treatments yielded highest hatch, while METH-5% and PG-5% yielded the most normal hatched embryos. Larval spinal torsion was higher for fresh than frozen-thawed sperm, where larvae with spinal torsion showed vertebral fusion and shape alterations during exogenous feeding. Both fresh and cryopreserved treatments showed abnormalities in caudal skeleton, while rates of defective yolk-sacs were higher for cryopreserved sperm, where larvae with defective yolks showed oversized yolk extension. Percentage of larvae with defective heads/eyes were also higher for cryopreserved sperm. Results show how frozen-thawed sperm impairs embryonic/larvae development and identifies frequency and position of abnormalities. Future studies should investigate how sperm DNA damage may have caused these alterations. This article is protected by copyright. All rights reserved.
Original languageEnglish
JournalDevelopmental Dynamics
Volume248
Issue number6
Pages (from-to)449-464
Number of pages16
ISSN1058-8388
DOIs
Publication statusPublished - 2019

Keywords

  • Cryopreservation
  • Deformity
  • Early life history
  • Embryology
  • Gene bank
  • Larval morphology

Cite this

@article{140db00b161c426c8b6fde581f9b9878,
title = "Outcomes of in vitro fertilization with frozen-thawed sperm: An analysis of post-thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish",
abstract = "Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are sired with fresh and frozen-thawed sperm. Cryopreserved sperm did not affect embryogenesis at 2- and 4-cell stages, but impaired embryonic development at 8-cell stage. Embryonic viability decreased at organogenesis, where only 34-49{\%} of embryos showed viability with frozen-thawed sperm. Hatching success and percentage of normal hatched embryos declined when fertilized with frozen-thawed sperm. Considering only frozen-thawed cells the DMSO-5{\%}, METH-5{\%}, and METH-10{\%} treatments yielded highest hatch, while METH-5{\%} and PG-5{\%} yielded the most normal hatched embryos. Larval spinal torsion was higher for fresh than frozen-thawed sperm, where larvae with spinal torsion showed vertebral fusion and shape alterations during exogenous feeding. Both fresh and cryopreserved treatments showed abnormalities in caudal skeleton, while rates of defective yolk-sacs were higher for cryopreserved sperm, where larvae with defective yolks showed oversized yolk extension. Percentage of larvae with defective heads/eyes were also higher for cryopreserved sperm. Results show how frozen-thawed sperm impairs embryonic/larvae development and identifies frequency and position of abnormalities. Future studies should investigate how sperm DNA damage may have caused these alterations. This article is protected by copyright. All rights reserved.",
keywords = "Cryopreservation, Deformity, Early life history, Embryology, Gene bank, Larval morphology",
author = "Vahid Zadmajid and Elham Falahipour and Edris Ghaderi and S{\o}rensen, {Sune Riis} and Butts, {Ian Anthony Ernest}",
year = "2019",
doi = "10.1002/dvdy.37",
language = "English",
volume = "248",
pages = "449--464",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "JohnWiley & Sons, Inc.",
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}

Outcomes of in vitro fertilization with frozen-thawed sperm: An analysis of post-thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish. / Zadmajid, Vahid; Falahipour, Elham; Ghaderi, Edris; Sørensen, Sune Riis; Butts, Ian Anthony Ernest.

In: Developmental Dynamics, Vol. 248, No. 6, 2019, p. 449-464.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Outcomes of in vitro fertilization with frozen-thawed sperm: An analysis of post-thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

AU - Zadmajid, Vahid

AU - Falahipour, Elham

AU - Ghaderi, Edris

AU - Sørensen, Sune Riis

AU - Butts, Ian Anthony Ernest

PY - 2019

Y1 - 2019

N2 - Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are sired with fresh and frozen-thawed sperm. Cryopreserved sperm did not affect embryogenesis at 2- and 4-cell stages, but impaired embryonic development at 8-cell stage. Embryonic viability decreased at organogenesis, where only 34-49% of embryos showed viability with frozen-thawed sperm. Hatching success and percentage of normal hatched embryos declined when fertilized with frozen-thawed sperm. Considering only frozen-thawed cells the DMSO-5%, METH-5%, and METH-10% treatments yielded highest hatch, while METH-5% and PG-5% yielded the most normal hatched embryos. Larval spinal torsion was higher for fresh than frozen-thawed sperm, where larvae with spinal torsion showed vertebral fusion and shape alterations during exogenous feeding. Both fresh and cryopreserved treatments showed abnormalities in caudal skeleton, while rates of defective yolk-sacs were higher for cryopreserved sperm, where larvae with defective yolks showed oversized yolk extension. Percentage of larvae with defective heads/eyes were also higher for cryopreserved sperm. Results show how frozen-thawed sperm impairs embryonic/larvae development and identifies frequency and position of abnormalities. Future studies should investigate how sperm DNA damage may have caused these alterations. This article is protected by copyright. All rights reserved.

AB - Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are sired with fresh and frozen-thawed sperm. Cryopreserved sperm did not affect embryogenesis at 2- and 4-cell stages, but impaired embryonic development at 8-cell stage. Embryonic viability decreased at organogenesis, where only 34-49% of embryos showed viability with frozen-thawed sperm. Hatching success and percentage of normal hatched embryos declined when fertilized with frozen-thawed sperm. Considering only frozen-thawed cells the DMSO-5%, METH-5%, and METH-10% treatments yielded highest hatch, while METH-5% and PG-5% yielded the most normal hatched embryos. Larval spinal torsion was higher for fresh than frozen-thawed sperm, where larvae with spinal torsion showed vertebral fusion and shape alterations during exogenous feeding. Both fresh and cryopreserved treatments showed abnormalities in caudal skeleton, while rates of defective yolk-sacs were higher for cryopreserved sperm, where larvae with defective yolks showed oversized yolk extension. Percentage of larvae with defective heads/eyes were also higher for cryopreserved sperm. Results show how frozen-thawed sperm impairs embryonic/larvae development and identifies frequency and position of abnormalities. Future studies should investigate how sperm DNA damage may have caused these alterations. This article is protected by copyright. All rights reserved.

KW - Cryopreservation

KW - Deformity

KW - Early life history

KW - Embryology

KW - Gene bank

KW - Larval morphology

U2 - 10.1002/dvdy.37

DO - 10.1002/dvdy.37

M3 - Journal article

VL - 248

SP - 449

EP - 464

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 6

ER -