Osteoblastic response to pectin nanocoating on titanium surfaces

Katarzyna Gurzawska, Rikke Svava, Yu Yihua, Kenneth Brian Haugshøj, Kai Dirscherl, Steven B. Levery, Inge Byg, Iben Damager, Martin Weiss Nielsen , Bodil Jørgensen, Niklas Rye Jørgensen, Klaus Gotfredsen

    Research output: Contribution to journalJournal articleResearchpeer-review


    Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro. © 2014 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    JournalMaterials Science and Engineering: A - Structural Materials: Properties, Microstructure and Processing
    Pages (from-to)117-125
    Number of pages9
    Publication statusPublished - 2014


    • Nanocoatings
    • Osseointegration
    • Osteoblasts
    • Rhamnogalacturonan-I
    • Surface properties
    • Titanium

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