Osmotic stress tolerance in semi-terrestrial tardigrades

Nanna W T Heidemann, Daniel K. Smith, Thomas L. Hygum, Lilian Stapane, Lykke K B Clausen, Aslak Jørgensen, Claus Hélix-Nielsen, Nadja Møbjerg

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Abstract

Little is known about ionic and osmotic stress tolerance in tardigrades. Here, we examine salt stress tolerance in Ramazzottius oberhaeuseri and Echiniscus testudo from Nivå (Denmark) and address whether limno-terrestrial tardigrades can enter a state of quiescence (osmobiosis) in the face of high external osmolyte concentrations. Direct transfers into NaCl solutions showed an upper tolerance level of around 600 mOsm kg−1 in R. oberhaeuseri and 200 mOsm kg−1 in E. testudo. During salt exposures, R. oberhaeuseri contracted into a ‘tun’, whereas E. testudo remained active leaving it more susceptible to acute effects of the ions. Further experiments focused on the more resilient R. oberhaeuseri, which entered a tun and readily regained activity when directly exposed to polyethylene glycol and sucrose of up to 872 ± 0 and 813 ± 3 mOsm kg−1, respectively, revealing a higher tolerance towards non-ionic osmolytes as compared to NaCl. Ramazzottius oberhaeuseri furthermore readily regained activity following gradual increases in non-ionic osmolytes and NaCl of up to 2434 ± 28 and 1905 ± 3 mOsm kg−1, respectively, showing that short-term acclimation promoted salt stress tolerance. Our results suggest that the limno-terrestrial R. oberhaeuseri enters a state of quiescence in the face of high external osmotic pressure and that it, in this state, is highly tolerant of ionic and osmotic stress.
Original languageEnglish
JournalZoological Journal of the Linnean Society
Volume178
Issue number4
Pages (from-to)912-918
Number of pages7
ISSN0024-4082
DOIs
Publication statusPublished - 2016

Keywords

  • Animal Science and Zoology
  • cryptobiosis
  • Ecdysozoa
  • extreme environments
  • ionic stress
  • osmobiosis

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