Organization and architecture of AggR-dependent promoters from Enteroaggregative Escherichia coli

Muhammad Yasir, Christopher Icke, Radwa Abdelwahab, James R. Haycocks, Rita E. Godfrey, Pavelas Sazinas, Mark J. Pallen, Ian R. Henderson, Stephen J. W. Busby*, Douglas F. Browning

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

48 Downloads (Pure)

Abstract

Enteroaggregative Escherichia coli (EAEC), is a diarrhoeagenic human pathogen commonly isolated from patients in both developing and industrialized countries. Pathogenic EAEC strains possess many virulence determinants, which are thought to be involved in causing disease, though, the exact mechanism by which EAEC causes diarrhoea is unclear. Typical EAEC strains possess the transcriptional regulator, AggR, which controls the expression of many virulence determinants, including the attachment adherence fimbriae (AAF) that are necessary for adherence to human gut epithelial cells. Here, using RNA-sequencing, we have investigated the AggR regulon from EAEC strain 042 and show that AggR regulates the transcription of genes on both the bacterial chromosome and the large virulence plasmid, pAA2. Due to the importance of fimbriae, we focused on the two AAF/II fimbrial gene clusters in EAEC 042 (afaB-aafCB and aafDA) and identified the promoter elements and AggR-binding sites required for fimbrial expression. In addition, we examined the organization of the fimbrial operon promoters from other important EAEC strains to understand the rules of AggR-dependent activation. Finally, we generated a series of semi-synthetic promoters to define the minimal sequence required for AggR-mediated activation and show that the correct positioning of a single AggR-binding site is sufficient to confer AggR-dependence. This article is protected by copyright. All rights reserved.
Original languageEnglish
JournalMolecular Microbiology
Volume111
Issue number2
Pages (from-to)534-551
Number of pages18
ISSN0950-382X
DOIs
Publication statusPublished - 2019

Keywords

  • EAEC
  • AggR
  • Bacterial gene regulation
  • Fimbriae
  • Promoter organization

Fingerprint

Dive into the research topics of 'Organization and architecture of AggR-dependent promoters from Enteroaggregative <i>Escherichia coli</i>'. Together they form a unique fingerprint.

Cite this