Optomagnetic Detection of MicroRNA Based on Duplex-Specific Nuclease-Assisted Target Recycling and Multilayer Core-Satellite Magnetic Superstructures

Bo Tian, Jing Ma, Zhen Qiu, Teresa Zardán Gómez de la Torre, Marco Donolato, Mikkel Fougt Hansen, Peter Svedlindh, Mattias Strömberg

    Research output: Contribution to journalJournal articleResearchpeer-review

    308 Downloads (Pure)

    Abstract

    Superstructural assembly of magnetic nanoparticles enables approaches to biosensing by combining specially tailored properties of superstructures and the particular advantages associated with a magnetic or optomagnetic read-out such as low background signal, easy manipulation, cost-efficiency, and potential for bioresponsive multiplexing. Herein, we demonstrate a sensitive and rapid miRNA detection method based on optomagnetic read-out, duplex-specific nuclease (DSN)-assisted target recycling, and the use of multilayer core-satellite magnetic superstructures. Triggered by the presence of target miRNA and DSN-assisted target recycling, the core-satellite magnetic superstructures release their "satellites" to the suspension, which subsequently can be quantified accurately in a low-cost and user-friendly optomagnetic setup. Target miRNAs are preserved in the cleaving reaction and can thereby trigger more cleavage and release of "satellites". For singleplex detection of let-7b, a linear detection range between 10 fM and 10 nM was observed, and a detection limit of 4.8 fM was obtained within a total assay time of 70 min. Multiplexing was achieved by releasing nanoparticles of different sizes in the presence of different miRNAs. The proposed method also has the advantages of single-nucleotide mismatch discrimination and the ability of quantification in a clinical sample matrix, thus holding great promise for miRNA routine multiplex diagnostics.
    Original languageEnglish
    JournalA C S Nano
    Volume11
    Pages (from-to)1798-1806
    ISSN1936-0851
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Core-satellite superstructures
    • Duplex-specific nuclease
    • Magnetic nanoparticles
    • miRNA detection
    • Optomagnetic bioassay

    Fingerprint Dive into the research topics of 'Optomagnetic Detection of MicroRNA Based on Duplex-Specific Nuclease-Assisted Target Recycling and Multilayer Core-Satellite Magnetic Superstructures'. Together they form a unique fingerprint.

    Cite this