Optofluidic microscope with 3D spatial resolution

Asger Laurberg Vig; Rodolphe Marie; Eric Jensen; Anders Kristensen

doi:10.1364/OE.18.004158

Optofluidic microscope with 3D spatial resolution

Asger Laurberg Vig, Rodolphe Marie, Eric Jensen, Anders Kristensen

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Abstract

This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1μm, 2.1μm, 3μm and 4μm is determined by the OFM. These results are bench marked against those obtained with a PFF device using conventional fluorescence microscope readout. The size separated microspheres are detected by OFM with an accuracy of ≤0.92μm. The position in the height of the channel and the velocity of the separated microspheres are detected with an accuracy of 1.4μm and 0.08 mm/s respectively. Throughout the measurements of the height and velocity distribution, the microspheres are observed to move towards the center of the channel in regard to its height.

Original language	English
Journal	Optics Express
Volume	18
Issue number	5
Pages (from-to)	4158-4169
ISSN	1094-4087
DOIs	https://doi.org/10.1364/OE.18.004158
Publication status	Published - 2010

Bibliographical note

This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-5-4158. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

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title = "Optofluidic microscope with 3D spatial resolution",

abstract = "This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1μm, 2.1μm, 3μm and 4μm is determined by the OFM. These results are bench marked against those obtained with a PFF device using conventional fluorescence microscope readout. The size separated microspheres are detected by OFM with an accuracy of ≤0.92μm. The position in the height of the channel and the velocity of the separated microspheres are detected with an accuracy of 1.4μm and 0.08 mm/s respectively. Throughout the measurements of the height and velocity distribution, the microspheres are observed to move towards the center of the channel in regard to its height.",

author = "Vig, {Asger Laurberg} and Rodolphe Marie and Eric Jensen and Anders Kristensen",

note = "This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-5-4158. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.",

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AU - Vig, Asger Laurberg

AU - Marie, Rodolphe

AU - Jensen, Eric

AU - Kristensen, Anders

N1 - This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-5-4158. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

PY - 2010

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N2 - This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1μm, 2.1μm, 3μm and 4μm is determined by the OFM. These results are bench marked against those obtained with a PFF device using conventional fluorescence microscope readout. The size separated microspheres are detected by OFM with an accuracy of ≤0.92μm. The position in the height of the channel and the velocity of the separated microspheres are detected with an accuracy of 1.4μm and 0.08 mm/s respectively. Throughout the measurements of the height and velocity distribution, the microspheres are observed to move towards the center of the channel in regard to its height.

AB - This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1μm, 2.1μm, 3μm and 4μm is determined by the OFM. These results are bench marked against those obtained with a PFF device using conventional fluorescence microscope readout. The size separated microspheres are detected by OFM with an accuracy of ≤0.92μm. The position in the height of the channel and the velocity of the separated microspheres are detected with an accuracy of 1.4μm and 0.08 mm/s respectively. Throughout the measurements of the height and velocity distribution, the microspheres are observed to move towards the center of the channel in regard to its height.

U2 - 10.1364/OE.18.004158

DO - 10.1364/OE.18.004158

M3 - Journal article

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VL - 18

SP - 4158

EP - 4169

JO - Optics Express

JF - Optics Express

IS - 5

ER -

Optofluidic microscope with 3D spatial resolution

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