Optimizing supercritical angle fluorescence structures in polymer microfluidic chips for highly sensitive pathogen detection

Trieu Nguyen; Tien Ngo Anh; Anders Wolff; Dang Duong Bang

Optimizing supercritical angle fluorescence structures in polymer microfluidic chips for highly sensitive pathogen detection

Trieu Nguyen, Tien Ngo Anh, Anders Wolff, Dang Duong Bang

National Food Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

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Abstract

We report an in-depth analysis and a fabrication method to precisely produce micro-sized arrays of supercritical angle fluorescence optical structures in disposal microfluidic polymer chips. This technique can be used for industrially massive production of disposal microfluidic polymer chips for pathogen detections with application in food safety and clinical diagnosis. For a demonstration, the chip is used to successfully detect E. coli with the limit of detection of 3.37 x 10²copies.

Original language	English
Title of host publication	Proceedings of the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Publication	Kaohsiung, Taiwan
Publication date	2018
Pages	2284-2285
ISBN (Electronic)	978-0-578-40530-8
Publication status	Published - 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China Duration: 11 Nov 2018 → 15 Nov 2018 Conference number: 22

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number	22
Location	Kaohsiung Exhibition Center
Country/Territory	Taiwan, Province of China
City	Kaohsiung
Period	11/11/2018 → 15/11/2018

Keywords

microfluidics
pathogen detections
diagnosis
polymer injection molding
fluorescence
PCR

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abstract = "We report an in-depth analysis and a fabrication method to precisely produce micro-sized arrays of supercritical angle fluorescence optical structures in disposal microfluidic polymer chips. This technique can be used for industrially massive production of disposal microfluidic polymer chips for pathogen detections with application in food safety and clinical diagnosis. For a demonstration, the chip is used to successfully detect E. coli with the limit of detection of 3.37 x 102 copies. ",

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Nguyen, T, Ngo Anh, T, Wolff, A & Bang, DD 2018, Optimizing supercritical angle fluorescence structures in polymer microfluidic chips for highly sensitive pathogen detection. in Proceedings of the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. Kaohsiung, Taiwan, pp. 2284-2285, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan, Province of China, 11/11/2018.

Optimizing supercritical angle fluorescence structures in polymer microfluidic chips for highly sensitive pathogen detection. / Nguyen, Trieu; Ngo Anh, Tien; Wolff, Anders et al.
Proceedings of the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. Kaohsiung, Taiwan, 2018. p. 2284-2285.

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

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KW - pathogen detections

KW - diagnosis

KW - polymer injection molding

KW - fluorescence

KW - PCR

M3 - Conference abstract in proceedings

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BT - Proceedings of the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences

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Optimizing supercritical angle fluorescence structures in polymer microfluidic chips for highly sensitive pathogen detection

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