Abstract
Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.
| Original language | English |
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| IPC | C12P 17/ 10 A I |
| Patent number | WO2017167866 |
| Filing date | 05/10/2017 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 19/05/2016 |
| Priority number | EP20160170405 |
| Publication status | Published - 5 Oct 2017 |