Optimization of the agar-gel method for isolation of migrating Ascaris suum larvae from the liver and lungs of pigs

I. Saeed, A. Roepstorff, T. Rasmussen, M. Hog, Gregers Jungersen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Experiments on use of an agar-gel method for recovery of migrating Ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. Subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1% agar and the larvae allowed to migrate out of the agar-gel into 0.9% NaCl at 38 degreesC. The results showed that within 3 h more than 88% of the recoverable larvae migrated out of the liver agar-gel and more than 83% of the obtained larvae migrated out of the lung agar-gel. The larvae were subsequently available in a very clean suspension which reduced the sample counting time. Blending the liver for 60 sec in a commercial blender showed significantly higher larvae recovery than blending for 30 sec. Addition of gentamycin to reduce bacterial growth during incubation, glucose to increase larval motility during migration or ice to increase sedimentation of migrated larvae did not influence larvae recovery significantly.
    Original languageEnglish
    JournalActa Veterinaria Scandinavica
    Volume42
    Issue number2
    Pages (from-to)279-286
    ISSN0044-605X
    DOIs
    Publication statusPublished - 2001

    Keywords

    • Ascaris suum
    • lungs
    • liver
    • agar-gel method
    • larva recovery

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