Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Helle Christine Ravn; Marianne L. Damstrup; Anne S. Meyer

doi:10.1016/j.bcab.2012.06.002

Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Helle Christine Ravn, Marianne L. Damstrup, Anne S. Meyer

Department of Chemical and Biochemical Engineering

Novozymes A/S

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.

Original language	English
Journal	Biocatalysis and Agricultural Biotechnology
Volume	1
Issue number	4
Pages (from-to)	273-279
ISSN	1878-8181
DOIs	https://doi.org/10.1016/j.bcab.2012.06.002
Publication status	Published - 2012

Keywords

Immobilized Candida antarctica lipase B
Fish oil concentrates
Omega-3 fatty acids
Central composite design
Esterification
Transesterification

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10.1016/j.bcab.2012.06.002

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title = "Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids",

abstract = "Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme{\texttrademark} 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.",

keywords = "Immobilized Candida antarctica lipase B, Fish oil concentrates, Omega-3 fatty acids, Central composite design, Esterification, Transesterification",

author = "Ravn, {Helle Christine} and Damstrup, {Marianne L.} and Meyer, {Anne S.}",

year = "2012",

doi = "10.1016/j.bcab.2012.06.002",

language = "English",

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journal = "Biocatalysis and Agricultural Biotechnology",

issn = "1878-8181",

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TY - JOUR

T1 - Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

AU - Ravn, Helle Christine

AU - Damstrup, Marianne L.

AU - Meyer, Anne S.

PY - 2012

Y1 - 2012

N2 - Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.

AB - Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.

KW - Immobilized Candida antarctica lipase B

KW - Fish oil concentrates

KW - Omega-3 fatty acids

KW - Central composite design

KW - Esterification

KW - Transesterification

U2 - 10.1016/j.bcab.2012.06.002

DO - 10.1016/j.bcab.2012.06.002

M3 - Journal article

SN - 1878-8181

VL - 1

SP - 273

EP - 279

JO - Biocatalysis and Agricultural Biotechnology

JF - Biocatalysis and Agricultural Biotechnology

IS - 4

ER -

Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Abstract

Keywords

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