Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Helle Christine Ravn, Marianne L. Damstrup, Anne S. Meyer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.
Original languageEnglish
JournalBiocatalysis and Agricultural Biotechnology
Volume1
Issue number4
Pages (from-to)273-279
ISSN1878-8181
DOIs
Publication statusPublished - 2012

Keywords

  • Immobilized Candida antarctica lipase B
  • Fish oil concentrates
  • Omega-3 fatty acids
  • Central composite design
  • Esterification
  • Transesterification

Fingerprint Dive into the research topics of 'Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids'. Together they form a unique fingerprint.

Cite this