Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Helle Christine Ravn, Marianne L. Damstrup, Anne S. Meyer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.
Original languageEnglish
JournalBiocatalysis and Agricultural Biotechnology
Volume1
Issue number4
Pages (from-to)273-279
ISSN1878-8181
DOIs
Publication statusPublished - 2012

Keywords

  • Immobilized Candida antarctica lipase B
  • Fish oil concentrates
  • Omega-3 fatty acids
  • Central composite design
  • Esterification
  • Transesterification

Cite this

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title = "Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids",
abstract = "Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95{\%} (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1{\%}w/w.",
keywords = "Immobilized Candida antarctica lipase B, Fish oil concentrates, Omega-3 fatty acids, Central composite design, Esterification, Transesterification",
author = "Ravn, {Helle Christine} and Damstrup, {Marianne L.} and Meyer, {Anne S.}",
year = "2012",
doi = "10.1016/j.bcab.2012.06.002",
language = "English",
volume = "1",
pages = "273--279",
journal = "Biocatalysis and Agricultural Biotechnology",
issn = "1878-8181",
publisher = "Elsevier",
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}

Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids. / Ravn, Helle Christine; Damstrup, Marianne L.; Meyer, Anne S.

In: Biocatalysis and Agricultural Biotechnology, Vol. 1, No. 4, 2012, p. 273-279.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

AU - Ravn, Helle Christine

AU - Damstrup, Marianne L.

AU - Meyer, Anne S.

PY - 2012

Y1 - 2012

N2 - Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.

AB - Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.

KW - Immobilized Candida antarctica lipase B

KW - Fish oil concentrates

KW - Omega-3 fatty acids

KW - Central composite design

KW - Esterification

KW - Transesterification

U2 - 10.1016/j.bcab.2012.06.002

DO - 10.1016/j.bcab.2012.06.002

M3 - Journal article

VL - 1

SP - 273

EP - 279

JO - Biocatalysis and Agricultural Biotechnology

JF - Biocatalysis and Agricultural Biotechnology

SN - 1878-8181

IS - 4

ER -