Optimization of Large Volume Injection for Improved Detection of Polycyclic Aromatic Hydrocarbons (PAH) in Mussels

Research output: Contribution to journalJournal article – Annual report year: 2008Researchpeer-review

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Optimization of Large Volume Injection for Improved Detection of Polycyclic Aromatic Hydrocarbons (PAH) in Mussels. / Duedahl-Olesen, Lene; Ghorbani, Faranak .

In: Polycyclic Aromatic Compounds, Vol. 28, No. 4-5, 2008, p. 282-301.

Research output: Contribution to journalJournal article – Annual report year: 2008Researchpeer-review

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@article{ea6d7ca9cf254dd189b2963167f28985,
title = "Optimization of Large Volume Injection for Improved Detection of Polycyclic Aromatic Hydrocarbons (PAH) in Mussels",
abstract = "Detection of PAH of six benzene rings is somewhat troublesome and lowering the limits of detection (LODs) for these compounds in food is necessary. For this purpose, we optimized a Programmable-Temperature-Vaporisation (PTV) injection with Large Volume Injection (LVI) with regard to the GC-MS detection of anthracene, benz[a]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene and dibenzo[a,e]pyrene. The optimization of PTV-LVI for GC-MS analysis included the choice of liner, solvent venting, splitless time, split flow and initial inlet temperature for injection of 25 L standard solution and spiked mussel samples. Samples were extracted with Accelerated Solvent Extraction (ASE) followed by two semi-automatic clean-up steps; gel permeation chromatography (GPC) on S-X3 and solid phase extraction (SPE) on pre-packed silica columns, prior to gas chromatography-mass spectrometry (GC-MS) detection. In comparison to traditional splitless injection, LODs were lowered for eighteen PAHs by the use of PTV-LVI ranging from 0.05 g kg-1to 1.0 g kg-1 fresh weight. In particular, the LOD of dibenzo[a,e]pyrene was improved by a factor of ten when using the validated PTV-LVI method.",
author = "Lene Duedahl-Olesen and Faranak Ghorbani",
year = "2008",
doi = "10.1080/10406630802378284",
language = "English",
volume = "28",
pages = "282--301",
journal = "Polycyclic Aromatic Compounds",
issn = "1040-6638",
publisher = "Taylor & Francis Inc",
number = "4-5",

}

RIS

TY - JOUR

T1 - Optimization of Large Volume Injection for Improved Detection of Polycyclic Aromatic Hydrocarbons (PAH) in Mussels

AU - Duedahl-Olesen, Lene

AU - Ghorbani, Faranak

PY - 2008

Y1 - 2008

N2 - Detection of PAH of six benzene rings is somewhat troublesome and lowering the limits of detection (LODs) for these compounds in food is necessary. For this purpose, we optimized a Programmable-Temperature-Vaporisation (PTV) injection with Large Volume Injection (LVI) with regard to the GC-MS detection of anthracene, benz[a]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene and dibenzo[a,e]pyrene. The optimization of PTV-LVI for GC-MS analysis included the choice of liner, solvent venting, splitless time, split flow and initial inlet temperature for injection of 25 L standard solution and spiked mussel samples. Samples were extracted with Accelerated Solvent Extraction (ASE) followed by two semi-automatic clean-up steps; gel permeation chromatography (GPC) on S-X3 and solid phase extraction (SPE) on pre-packed silica columns, prior to gas chromatography-mass spectrometry (GC-MS) detection. In comparison to traditional splitless injection, LODs were lowered for eighteen PAHs by the use of PTV-LVI ranging from 0.05 g kg-1to 1.0 g kg-1 fresh weight. In particular, the LOD of dibenzo[a,e]pyrene was improved by a factor of ten when using the validated PTV-LVI method.

AB - Detection of PAH of six benzene rings is somewhat troublesome and lowering the limits of detection (LODs) for these compounds in food is necessary. For this purpose, we optimized a Programmable-Temperature-Vaporisation (PTV) injection with Large Volume Injection (LVI) with regard to the GC-MS detection of anthracene, benz[a]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene and dibenzo[a,e]pyrene. The optimization of PTV-LVI for GC-MS analysis included the choice of liner, solvent venting, splitless time, split flow and initial inlet temperature for injection of 25 L standard solution and spiked mussel samples. Samples were extracted with Accelerated Solvent Extraction (ASE) followed by two semi-automatic clean-up steps; gel permeation chromatography (GPC) on S-X3 and solid phase extraction (SPE) on pre-packed silica columns, prior to gas chromatography-mass spectrometry (GC-MS) detection. In comparison to traditional splitless injection, LODs were lowered for eighteen PAHs by the use of PTV-LVI ranging from 0.05 g kg-1to 1.0 g kg-1 fresh weight. In particular, the LOD of dibenzo[a,e]pyrene was improved by a factor of ten when using the validated PTV-LVI method.

U2 - 10.1080/10406630802378284

DO - 10.1080/10406630802378284

M3 - Journal article

VL - 28

SP - 282

EP - 301

JO - Polycyclic Aromatic Compounds

JF - Polycyclic Aromatic Compounds

SN - 1040-6638

IS - 4-5

ER -