Optimization of 3D-printed microstructures for investigating the properties of the mucus biobarrier

Ada-Ioana Bunea*, Mogens Havsteen Jakobsen, Einstom Engay, Andrew Rafael Bañas, Jesper Glückstad

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In order to overcome the mucus biobarrier for drug delivery purposes, a better understanding of the interactions between mucus and the drug carrier is needed. We propose optical catapulting of 3D-printed microstructures with tailored shape and surface chemistry as a means to study the interaction filtering properties of a model mucus biobarrier in dynamic conditions. Using twophoton polymerization, we fabricate microstructures with a resolution of approximately 200 nm. We introduce amino functional groups on the surface of the IP-L 780-derived polymer in a single step process via UV-assisted functionalization with an anthraquinone amine photolinker. Our optical catapulting system relies on Generalized Phase Contrast for beam shaping and it allows us to manipulate microstructures over a distance of 250 µm, similar to the mucus layer thickness in the upper part of the lower human intestine. This work is part of an ongoing endeavor to establish Light Robotics as a valuable toolbox for biomedical research.
Original languageEnglish
JournalMicro and Nano Engineering
Volume2
Pages (from-to)41-47
ISSN2590-0072
DOIs
Publication statusPublished - 2019

Keywords

  • Optical catapulting
  • Two-Photon Polymerization
  • Surface modification.
  • Generalized Phase Contrast
  • Light Robotics
  • Mucus biobarrier

Cite this

@article{c90c0c535e364f3eb1f36547d7187429,
title = "Optimization of 3D-printed microstructures for investigating the properties of the mucus biobarrier",
abstract = "In order to overcome the mucus biobarrier for drug delivery purposes, a better understanding of the interactions between mucus and the drug carrier is needed. We propose optical catapulting of 3D-printed microstructures with tailored shape and surface chemistry as a means to study the interaction filtering properties of a model mucus biobarrier in dynamic conditions. Using twophoton polymerization, we fabricate microstructures with a resolution of approximately 200 nm. We introduce amino functional groups on the surface of the IP-L 780-derived polymer in a single step process via UV-assisted functionalization with an anthraquinone amine photolinker. Our optical catapulting system relies on Generalized Phase Contrast for beam shaping and it allows us to manipulate microstructures over a distance of 250 µm, similar to the mucus layer thickness in the upper part of the lower human intestine. This work is part of an ongoing endeavor to establish Light Robotics as a valuable toolbox for biomedical research.",
keywords = "Optical catapulting, Two-Photon Polymerization, Surface modification., Generalized Phase Contrast, Light Robotics, Mucus biobarrier",
author = "Ada-Ioana Bunea and Jakobsen, {Mogens Havsteen} and Einstom Engay and Ba{\~n}as, {Andrew Rafael} and Jesper Gl{\"u}ckstad",
year = "2019",
doi = "10.1016/j.mne.2018.12.004",
language = "English",
volume = "2",
pages = "41--47",
journal = "Micro and Nano Engineering",
issn = "2590-0072",
publisher = "Elsevier",

}

Optimization of 3D-printed microstructures for investigating the properties of the mucus biobarrier. / Bunea, Ada-Ioana; Jakobsen, Mogens Havsteen; Engay, Einstom; Bañas, Andrew Rafael; Glückstad, Jesper.

In: Micro and Nano Engineering, Vol. 2, 2019, p. 41-47.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Optimization of 3D-printed microstructures for investigating the properties of the mucus biobarrier

AU - Bunea, Ada-Ioana

AU - Jakobsen, Mogens Havsteen

AU - Engay, Einstom

AU - Bañas, Andrew Rafael

AU - Glückstad, Jesper

PY - 2019

Y1 - 2019

N2 - In order to overcome the mucus biobarrier for drug delivery purposes, a better understanding of the interactions between mucus and the drug carrier is needed. We propose optical catapulting of 3D-printed microstructures with tailored shape and surface chemistry as a means to study the interaction filtering properties of a model mucus biobarrier in dynamic conditions. Using twophoton polymerization, we fabricate microstructures with a resolution of approximately 200 nm. We introduce amino functional groups on the surface of the IP-L 780-derived polymer in a single step process via UV-assisted functionalization with an anthraquinone amine photolinker. Our optical catapulting system relies on Generalized Phase Contrast for beam shaping and it allows us to manipulate microstructures over a distance of 250 µm, similar to the mucus layer thickness in the upper part of the lower human intestine. This work is part of an ongoing endeavor to establish Light Robotics as a valuable toolbox for biomedical research.

AB - In order to overcome the mucus biobarrier for drug delivery purposes, a better understanding of the interactions between mucus and the drug carrier is needed. We propose optical catapulting of 3D-printed microstructures with tailored shape and surface chemistry as a means to study the interaction filtering properties of a model mucus biobarrier in dynamic conditions. Using twophoton polymerization, we fabricate microstructures with a resolution of approximately 200 nm. We introduce amino functional groups on the surface of the IP-L 780-derived polymer in a single step process via UV-assisted functionalization with an anthraquinone amine photolinker. Our optical catapulting system relies on Generalized Phase Contrast for beam shaping and it allows us to manipulate microstructures over a distance of 250 µm, similar to the mucus layer thickness in the upper part of the lower human intestine. This work is part of an ongoing endeavor to establish Light Robotics as a valuable toolbox for biomedical research.

KW - Optical catapulting

KW - Two-Photon Polymerization

KW - Surface modification.

KW - Generalized Phase Contrast

KW - Light Robotics

KW - Mucus biobarrier

U2 - 10.1016/j.mne.2018.12.004

DO - 10.1016/j.mne.2018.12.004

M3 - Journal article

VL - 2

SP - 41

EP - 47

JO - Micro and Nano Engineering

JF - Micro and Nano Engineering

SN - 2590-0072

ER -