Optimised deconjugation of androgenic steroid conjugates in bovine urine

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

Documents

DOI

View graph of relations

After administration of steroids to animals the steroids are partially metabolised in the liver and kidney to phase 2 metabolites, i.e., glucuronic acid or sulphate conjugates. During analysis these conjugated metabolites are normally deconjugated enzymatically with aryl sulphatase and glucuronidase resulting in free steroids in the extract. It is well known that some sulphates are not deconjugated using aryl sulphatase; instead, for example, solvolysis can be used for deconjugation of these aliphatic sulphates. The effectiveness of solvolysis on androgenic steroid sulphates was tested with selected aliphatic steroid sulphates (boldenone sulphate, nortestosteron sulphate and testosterone sulphate), and the method was validated for analysis of androgenic steroids in bovine urine using free steroids, steroid sulphates and steroid glucuronides as standards. Glucuronidase and sulphuric acid in ethyl acetate were used for deconjugation and the extract was purified by solid-phase extraction. The final extract was evaporated to dryness, re-dissolved and analysed by LC-MS/MS.
Original languageEnglish
JournalFood Additives & Contaminants : Part A - Chemistry, Analysis, Control, Exposure & Risk Assessment
Volume34
Issue number4
Pages (from-to)482-488
ISSN1944-0049
DOIs
Publication statusPublished - 2017
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • LC-MS/MS, Steroid conjugates, deconjugation, solvolysis

Download statistics

No data available

ID: 128720645