Optical two-beam trap in a polymer microfluidic chip

Marta Espina Palanco (Invited author), Darmin Catak (Invited author), Rodolphe Marie (Invited author), Marco Matteucci (Invited author), Brian Bilenberg (Invited author), Anders Kristensen (Invited author), Kirstine Berg-Sørensen (Invited author)

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

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Abstract

An optical two-beam trap, composed from two counter propagating laser beams, is an interesting setup due to the ability of the system to trap, hold, and stretch soft biological objects like vesicles or single cells. Because of this functionality, the system was also named "the optical stretcher" by Jochen Guck, Josep Käs and co-workers some 15 years ago. In a favorable setup, the two opposing laser beams meet with equal intensities in the middle of a fluidic channel in which cells may flow past, be trapped, stretched, and allowed to move on, giving the promise of a high throughput device. Yet, single beam optical traps, aka optical tweezers, by far outnumber the existing optical stretchers in research labs throughout the world. The ability to easily construct an optical stretcher setup in a low-cost material would possibly imply more frequent use of the optical stretching technique. Here, we will outline the design, the production procedures, and results obtained in a fiber-based experimental setup built within an injection molded microfluidic polymer chip. The microfluidic chip is constructed with a three layer technology in which we ensure both horizontal and vertical focusing of the cells we wish to trap, thereby preventing too many cells to flow below the line of focus of the two counter propagating laser beams that are positioned perpendicular to the direction of flow of the cells. Results will be compared to that from other designs from previous work in the group.
Original languageEnglish
Title of host publicationProceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII
Number of pages10
Volume9922
PublisherSPIE - International Society for Optical Engineering
Publication date2016
DOIs
Publication statusPublished - 2016
EventOptical Trapping and Optical Micromanipulation XIII - San Diego, CA, United States
Duration: 28 Aug 20161 Sep 2016
Conference number: 13

Conference

ConferenceOptical Trapping and Optical Micromanipulation XIII
Number13
CountryUnited States
CitySan Diego, CA
Period28/08/201601/09/2016
SeriesS P I E - International Society for Optical Engineering. Proceedings
Volume9922
ISSN0277-786X

Keywords

  • Optical trapping
  • Fiber-based optical trap
  • Polymer injection molding
  • Hydrodynamic focusing

Cite this

Palanco, M. E., Catak, D., Marie, R., Matteucci, M., Bilenberg, B., Kristensen, A., & Berg-Sørensen, K. (2016). Optical two-beam trap in a polymer microfluidic chip. In Proceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII (Vol. 9922). SPIE - International Society for Optical Engineering. S P I E - International Society for Optical Engineering. Proceedings, Vol.. 9922 https://doi.org/10.1117/12.2236465
Palanco, Marta Espina ; Catak, Darmin ; Marie, Rodolphe ; Matteucci, Marco ; Bilenberg, Brian ; Kristensen, Anders ; Berg-Sørensen, Kirstine. / Optical two-beam trap in a polymer microfluidic chip. Proceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII . Vol. 9922 SPIE - International Society for Optical Engineering, 2016. (S P I E - International Society for Optical Engineering. Proceedings, Vol. 9922).
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keywords = "Optical trapping, Fiber-based optical trap, Polymer injection molding, Hydrodynamic focusing",
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Palanco, ME, Catak, D, Marie, R, Matteucci, M, Bilenberg, B, Kristensen, A & Berg-Sørensen, K 2016, Optical two-beam trap in a polymer microfluidic chip. in Proceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII . vol. 9922, SPIE - International Society for Optical Engineering, S P I E - International Society for Optical Engineering. Proceedings, vol. 9922, Optical Trapping and Optical Micromanipulation XIII, San Diego, CA, United States, 28/08/2016. https://doi.org/10.1117/12.2236465

Optical two-beam trap in a polymer microfluidic chip. / Palanco, Marta Espina (Invited author); Catak, Darmin (Invited author); Marie, Rodolphe (Invited author); Matteucci, Marco (Invited author); Bilenberg, Brian (Invited author); Kristensen, Anders (Invited author); Berg-Sørensen, Kirstine (Invited author).

Proceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII . Vol. 9922 SPIE - International Society for Optical Engineering, 2016. (S P I E - International Society for Optical Engineering. Proceedings, Vol. 9922).

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

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AU - Catak, Darmin

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AU - Kristensen, Anders

AU - Berg-Sørensen, Kirstine

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AB - An optical two-beam trap, composed from two counter propagating laser beams, is an interesting setup due to the ability of the system to trap, hold, and stretch soft biological objects like vesicles or single cells. Because of this functionality, the system was also named "the optical stretcher" by Jochen Guck, Josep Käs and co-workers some 15 years ago. In a favorable setup, the two opposing laser beams meet with equal intensities in the middle of a fluidic channel in which cells may flow past, be trapped, stretched, and allowed to move on, giving the promise of a high throughput device. Yet, single beam optical traps, aka optical tweezers, by far outnumber the existing optical stretchers in research labs throughout the world. The ability to easily construct an optical stretcher setup in a low-cost material would possibly imply more frequent use of the optical stretching technique. Here, we will outline the design, the production procedures, and results obtained in a fiber-based experimental setup built within an injection molded microfluidic polymer chip. The microfluidic chip is constructed with a three layer technology in which we ensure both horizontal and vertical focusing of the cells we wish to trap, thereby preventing too many cells to flow below the line of focus of the two counter propagating laser beams that are positioned perpendicular to the direction of flow of the cells. Results will be compared to that from other designs from previous work in the group.

KW - Optical trapping

KW - Fiber-based optical trap

KW - Polymer injection molding

KW - Hydrodynamic focusing

U2 - 10.1117/12.2236465

DO - 10.1117/12.2236465

M3 - Article in proceedings

VL - 9922

T3 - S P I E - International Society for Optical Engineering. Proceedings

BT - Proceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII

PB - SPIE - International Society for Optical Engineering

ER -

Palanco ME, Catak D, Marie R, Matteucci M, Bilenberg B, Kristensen A et al. Optical two-beam trap in a polymer microfluidic chip. In Proceedings of SPIE 9922, Optical Trapping and Optical Micromanipulation XIII . Vol. 9922. SPIE - International Society for Optical Engineering. 2016. (S P I E - International Society for Optical Engineering. Proceedings, Vol. 9922). https://doi.org/10.1117/12.2236465