Optical stretching on chip with acoustophoretic prefocusing

Maria Khoury Arvelo, L. Laub Busk, Henrik Bruus, Kirstine Berg-Sørensen, Rune Barnkob, Peter Tidemand-Lichtenberg

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

We demonstrate the use of a two-beam optical trap (an optical stretcher) in a low-cost microuidic system with the purpose of measuring the mechanical properties of cells and vesicles. Delivery of micrometer-sized particles and cells to the optical stretcher is obtained by acoustophoretic prefocusing. This focusing mechanism aims for target particles to always ow in the correct height relative to the optical stretcher, and is induced by a piezo-electric ultrasound transducer attached underneath the chip and driven at a frequency leading to a vertical standing ultrasound wave in the microchannel. Trapping and manipulation is demonstrated for dielectric beads. In addition, we show trapping, manipulation and stretching of red blood cells and vesicles, whereby we extract the elastic properties of these objects. Our design points towards the construction of a low-cost, high-throughput lab-on-a-chip device for measurement of mechanical properties of cells and vesicles.
Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering : Optical Trapping and Optical Micromanipulation IX
Number of pages11
Volume8458
Publication date2012
DOIs
Publication statusPublished - 2012
Event2012 Optics+Photonics : Optical Trapping and Optical Micromanipulation IX - San Diego Convention Center, San Diego, CA, United States
Duration: 12 Aug 201216 Aug 2012

Conference

Conference2012 Optics+Photonics : Optical Trapping and Optical Micromanipulation IX
LocationSan Diego Convention Center
CountryUnited States
CitySan Diego, CA
Period12/08/201216/08/2012

Keywords

  • Bioassay
  • Biochips
  • Biomechanics
  • Elasticity
  • Micromanipulators
  • Stretchers
  • Ultrasonic transducers
  • Molecular biology

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