Optical cell sorting with multiple imaging modalities

Andrew Banas, Caro Carrissemoux, Darwin Palima, Jesper Glückstad

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Abstract

Early detection of diseases can save lives. Hence, there is emphasis in sorting rare disease-indicating cells within small dilute quantities such as in the confines of lab-on-a-chip devices. However, before diseased cells can be studied in isolation, it is necessary to identify them against normal healthy cells. With the richness of visual information, a lot of microscopy techniques have been developed and have been crucial in biological studies. To utilize their complementary advantages we adopt both fluorescence and brightfield imaging in our optical cell sorter. Brightfield imaging has the advantage of being non-invasive, thus maintaining cell viability. Fluorescence imaging, on the other hand, takes advantages of the chemical specificity of fluorescence markers and can validate machine vision results from brightfield images. Visually identified cells are sorted using optical manipulation techniques. Scattering forces from beams actuated via efficient phase-only efficient modulation has been adopted. This has lowered the required power for sorting cells to a tenth of our previous approach, and also makes the cell sorter safer for use in clinical settings. With the versatility of dynamically programmable phase spatial light modulators, a plurality of light shaping techniques, including hybrid approaches, can be utilized in cell sorting.
Original languageEnglish
Title of host publicationProceedings of SPIE : Complex Light and Optical Forces XI
Number of pages6
Volume10120
PublisherSPIE - The International Society for Optical Engineering
Publication date2017
Article number1012018
DOIs
Publication statusPublished - 2017
EventComplex Light and Optical Forces XI: SPIE Conference 10120 - The Moscone Center, San Francisco, United States
Duration: 31 Jan 20172 Feb 2017

Conference

ConferenceComplex Light and Optical Forces XI
LocationThe Moscone Center
CountryUnited States
CitySan Francisco
Period31/01/201702/02/2017
SeriesProceedings of SPIE, the International Society for Optical Engineering
Volume10120
ISSN0277-786X

Bibliographical note

Copyright 2017 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

  • Cell sorting
  • Fluorescence microscopy
  • Microscopy
  • Phase-only light modulation
  • Optical trapping and manipulation
  • Machine vision
  • Microfluidics

Cite this

Banas, A., Carrissemoux, C., Palima, D., & Glückstad, J. (2017). Optical cell sorting with multiple imaging modalities. In Proceedings of SPIE: Complex Light and Optical Forces XI (Vol. 10120). [1012018] SPIE - The International Society for Optical Engineering. Proceedings of SPIE, the International Society for Optical Engineering, Vol.. 10120 https://doi.org/10.1117/12.2254358