Optical catapulting of microspheres in mucus models—toward overcoming the mucus biobarrier

Ada-Ioana Bunea, Manto Chouliara, Andrew Rafael Bañas, Einstom Engay, Jesper Glückstad*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The generalized phase contrast method is employed as an efficient "phase-only" laser beam-shaping technique in an optical setup built for catapulting microspheres through simple mucus models. The influence of the laser power and mucin concentration on the motion of the microspheres is investigated in terms of instant and average velocities on a 250-μm trajectory, corresponding to the mucus thickness in the human gastrointestinal tract. Increasing the laser power leads to higher velocities in all the tested samples, while increasing the mucin concentration leads to significant velocity decrease for similar laser input power. However, velocities of up to 95  μm  ·  s  -  1 are demonstrated in a 5% mucin simple mucus model using our catapulting system. This study contributes to understanding and overcoming the challenges of optical manipulation in mucus models. This paves the way for efficient optical manipulation of three-dimensional-printed light-controlled microtools with the ability to penetrate the mucus biobarrier for in vitro drug-delivery studies.

Original languageEnglish
Article number035001
JournalJournal of Biomedical Optics
Volume24
Issue number3
Number of pages9
ISSN1083-3668
DOIs
Publication statusPublished - 2019

Keywords

  • Optical catapulting
  • Generalized phase contrast
  • Particle tracking
  • Mucus model
  • Light Robotics

Cite this

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title = "Optical catapulting of microspheres in mucus models—toward overcoming the mucus biobarrier",
abstract = "The generalized phase contrast method is employed as an efficient {"}phase-only{"} laser beam-shaping technique in an optical setup built for catapulting microspheres through simple mucus models. The influence of the laser power and mucin concentration on the motion of the microspheres is investigated in terms of instant and average velocities on a 250-μm trajectory, corresponding to the mucus thickness in the human gastrointestinal tract. Increasing the laser power leads to higher velocities in all the tested samples, while increasing the mucin concentration leads to significant velocity decrease for similar laser input power. However, velocities of up to 95  μm  ·  s  -  1 are demonstrated in a 5{\%} mucin simple mucus model using our catapulting system. This study contributes to understanding and overcoming the challenges of optical manipulation in mucus models. This paves the way for efficient optical manipulation of three-dimensional-printed light-controlled microtools with the ability to penetrate the mucus biobarrier for in vitro drug-delivery studies.",
keywords = "Optical catapulting, Generalized phase contrast, Particle tracking, Mucus model, Light Robotics",
author = "Ada-Ioana Bunea and Manto Chouliara and Ba{\~n}as, {Andrew Rafael} and Einstom Engay and Jesper Gl{\"u}ckstad",
year = "2019",
doi = "10.1117/1.JBO.24.3.035001",
language = "English",
volume = "24",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "S P I E - International Society for Optical Engineering",
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Optical catapulting of microspheres in mucus models—toward overcoming the mucus biobarrier. / Bunea, Ada-Ioana; Chouliara, Manto; Bañas, Andrew Rafael; Engay, Einstom; Glückstad, Jesper.

In: Journal of Biomedical Optics, Vol. 24, No. 3, 035001 , 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Optical catapulting of microspheres in mucus models—toward overcoming the mucus biobarrier

AU - Bunea, Ada-Ioana

AU - Chouliara, Manto

AU - Bañas, Andrew Rafael

AU - Engay, Einstom

AU - Glückstad, Jesper

PY - 2019

Y1 - 2019

N2 - The generalized phase contrast method is employed as an efficient "phase-only" laser beam-shaping technique in an optical setup built for catapulting microspheres through simple mucus models. The influence of the laser power and mucin concentration on the motion of the microspheres is investigated in terms of instant and average velocities on a 250-μm trajectory, corresponding to the mucus thickness in the human gastrointestinal tract. Increasing the laser power leads to higher velocities in all the tested samples, while increasing the mucin concentration leads to significant velocity decrease for similar laser input power. However, velocities of up to 95  μm  ·  s  -  1 are demonstrated in a 5% mucin simple mucus model using our catapulting system. This study contributes to understanding and overcoming the challenges of optical manipulation in mucus models. This paves the way for efficient optical manipulation of three-dimensional-printed light-controlled microtools with the ability to penetrate the mucus biobarrier for in vitro drug-delivery studies.

AB - The generalized phase contrast method is employed as an efficient "phase-only" laser beam-shaping technique in an optical setup built for catapulting microspheres through simple mucus models. The influence of the laser power and mucin concentration on the motion of the microspheres is investigated in terms of instant and average velocities on a 250-μm trajectory, corresponding to the mucus thickness in the human gastrointestinal tract. Increasing the laser power leads to higher velocities in all the tested samples, while increasing the mucin concentration leads to significant velocity decrease for similar laser input power. However, velocities of up to 95  μm  ·  s  -  1 are demonstrated in a 5% mucin simple mucus model using our catapulting system. This study contributes to understanding and overcoming the challenges of optical manipulation in mucus models. This paves the way for efficient optical manipulation of three-dimensional-printed light-controlled microtools with the ability to penetrate the mucus biobarrier for in vitro drug-delivery studies.

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KW - Particle tracking

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