Optical and hydrodynamic stretching of single cells from blood

Henrik Thirstrup, Tony B. Rungling, Mustafa Zyad Khalil Al-Hamdani, Ragavan Pathanchalinathan, Morten Hanefeld Dziegiel, Anders Kristensen, Rodolphe Marie, Kirstine Berg-Sørensen

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Abstract

Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.
Original languageEnglish
JournalOptics Infobase Conference Papers
Volume2017
Number of pages3
ISSN2162-2701
DOIs
Publication statusPublished - 2017
EventOSA Biophotonics Congress: Optical Trapping Applications 2017: Optics in the Life Sciences - Sheraton San Diego Hotel & Marina, San Diego, United States
Duration: 2 Apr 20175 Apr 2017
http://www.osa.org/en-us/meetings/osa_meetings/optics_in_the_life_sciences/optical_trapping_applications_(1)/

Conference

ConferenceOSA Biophotonics Congress: Optical Trapping Applications 2017
LocationSheraton San Diego Hotel & Marina
CountryUnited States
CitySan Diego
Period02/04/201705/04/2017
Internet address

Cite this

Thirstrup, Henrik ; Rungling, Tony B. ; Khalil Al-Hamdani, Mustafa Zyad ; Pathanchalinathan, Ragavan ; Dziegiel, Morten Hanefeld ; Kristensen, Anders ; Marie, Rodolphe ; Berg-Sørensen, Kirstine. / Optical and hydrodynamic stretching of single cells from blood. In: Optics Infobase Conference Papers. 2017 ; Vol. 2017.
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Optical and hydrodynamic stretching of single cells from blood. / Thirstrup, Henrik; Rungling, Tony B.; Khalil Al-Hamdani, Mustafa Zyad; Pathanchalinathan, Ragavan; Dziegiel, Morten Hanefeld; Kristensen, Anders; Marie, Rodolphe ; Berg-Sørensen, Kirstine.

In: Optics Infobase Conference Papers, Vol. 2017, 2017.

Research output: Contribution to journalConference articleResearchpeer-review

TY - GEN

T1 - Optical and hydrodynamic stretching of single cells from blood

AU - Thirstrup, Henrik

AU - Rungling, Tony B.

AU - Khalil Al-Hamdani, Mustafa Zyad

AU - Pathanchalinathan, Ragavan

AU - Dziegiel, Morten Hanefeld

AU - Kristensen, Anders

AU - Marie, Rodolphe

AU - Berg-Sørensen, Kirstine

PY - 2017

Y1 - 2017

N2 - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

AB - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

U2 - 10.1364/OTA.2017.OtM4E.1

DO - 10.1364/OTA.2017.OtM4E.1

M3 - Conference article

VL - 2017

JO - Optics Infobase Conference Papers

JF - Optics Infobase Conference Papers

SN - 2162-2701

ER -

Thirstrup H, Rungling TB, Khalil Al-Hamdani MZ, Pathanchalinathan R, Dziegiel MH, Kristensen A et al. Optical and hydrodynamic stretching of single cells from blood. Optics Infobase Conference Papers. 2017;2017. https://doi.org/10.1364/OTA.2017.OtM4E.1