Abstract
| Original language | English |
|---|---|
| Journal | Optics Infobase Conference Papers |
| Volume | 2017 |
| Number of pages | 3 |
| ISSN | 2162-2701 |
| DOIs | |
| Publication status | Published - 2017 |
| Event | OSA Biophotonics Congress: Optical Trapping Applications 2017: Optics in the Life Sciences - Sheraton San Diego Hotel & Marina, San Diego, United States Duration: 2 Apr 2017 → 5 Apr 2017 http://www.osa.org/en-us/meetings/osa_meetings/optics_in_the_life_sciences/optical_trapping_applications_(1)/ |
Conference
| Conference | OSA Biophotonics Congress: Optical Trapping Applications 2017 |
|---|---|
| Location | Sheraton San Diego Hotel & Marina |
| Country | United States |
| City | San Diego |
| Period | 02/04/2017 → 05/04/2017 |
| Internet address |
Cite this
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Optical and hydrodynamic stretching of single cells from blood. / Thirstrup, Henrik; Rungling, Tony B.; Khalil Al-Hamdani, Mustafa Zyad; Pathanchalinathan, Ragavan; Dziegiel, Morten Hanefeld; Kristensen, Anders; Marie, Rodolphe ; Berg-Sørensen, Kirstine.
In: Optics Infobase Conference Papers, Vol. 2017, 2017.Research output: Contribution to journal › Conference article › Research › peer-review
TY - GEN
T1 - Optical and hydrodynamic stretching of single cells from blood
AU - Thirstrup, Henrik
AU - Rungling, Tony B.
AU - Khalil Al-Hamdani, Mustafa Zyad
AU - Pathanchalinathan, Ragavan
AU - Dziegiel, Morten Hanefeld
AU - Kristensen, Anders
AU - Marie, Rodolphe
AU - Berg-Sørensen, Kirstine
PY - 2017
Y1 - 2017
N2 - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.
AB - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.
U2 - 10.1364/OTA.2017.OtM4E.1
DO - 10.1364/OTA.2017.OtM4E.1
M3 - Conference article
VL - 2017
JO - Optics Infobase Conference Papers
JF - Optics Infobase Conference Papers
SN - 2162-2701
ER -