Oocyte and egg quality indicators in European eel: Lipid droplet coalescence and fatty acid composition

Filipa da Silva*, Charlotte Jacobsen, Elin Kjørsvik, Josianne Gatt Støttrup, Jonna Tomkiewicz

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

During European eel assisted reproduction, timely administration of hormones that induce oocyte maturation and ovulation is a major factor influencing subsequent egg quality. This treatment commonly comprises one injection of fish pituitary extract (PE) as a primer followed by a 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) injection. In this context, the present study aimed at optimizing timing of the dual hormone administration by applying a lipid droplet-based oocyte maturation scale, previously developed for Japanese eel to determine the maturational status of each female. Using wild-caught female eels, the potential effect of female size, egg fatty acid composition and dry weight on egg quality was also analyzed. Larval survival at 3 days post hatch was used to differentiate High- and Low-quality egg batches. Results showed that lipid droplet diameter was significantly smaller in High-quality eggs than in Low-quality egg batches, indicating that females producing High-quality eggs received the PE primer and DHP generally at an earlier developmental stage than those producing Low-quality batches. These results confirm that oocyte lipid droplet diameter is a useful indicator of female maturational status for optimization of induction of oocyte maturation and ovulation in European eel. Additional parameters, including female size, egg fatty acid composition and dry weight, were similar between high and low quality egg batches. This insight regarding the fatty acid composition of eggs obtained from wild-caught female eels may help advancing the development of tailored diets for increased reproductive success of farmed broodstock
Original languageEnglish
JournalAquaculture
Volume496
Pages (from-to)30-38
ISSN0044-8486
DOIs
Publication statusPublished - 2018

Cite this

@article{f11ddd47ad5c4e45847261b17e53e959,
title = "Oocyte and egg quality indicators in European eel: Lipid droplet coalescence and fatty acid composition",
abstract = "During European eel assisted reproduction, timely administration of hormones that induce oocyte maturation and ovulation is a major factor influencing subsequent egg quality. This treatment commonly comprises one injection of fish pituitary extract (PE) as a primer followed by a 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) injection. In this context, the present study aimed at optimizing timing of the dual hormone administration by applying a lipid droplet-based oocyte maturation scale, previously developed for Japanese eel to determine the maturational status of each female. Using wild-caught female eels, the potential effect of female size, egg fatty acid composition and dry weight on egg quality was also analyzed. Larval survival at 3 days post hatch was used to differentiate High- and Low-quality egg batches. Results showed that lipid droplet diameter was significantly smaller in High-quality eggs than in Low-quality egg batches, indicating that females producing High-quality eggs received the PE primer and DHP generally at an earlier developmental stage than those producing Low-quality batches. These results confirm that oocyte lipid droplet diameter is a useful indicator of female maturational status for optimization of induction of oocyte maturation and ovulation in European eel. Additional parameters, including female size, egg fatty acid composition and dry weight, were similar between high and low quality egg batches. This insight regarding the fatty acid composition of eggs obtained from wild-caught female eels may help advancing the development of tailored diets for increased reproductive success of farmed broodstock",
author = "{da Silva}, Filipa and Charlotte Jacobsen and Elin Kj{\o}rsvik and St{\o}ttrup, {Josianne Gatt} and Jonna Tomkiewicz",
year = "2018",
doi = "10.1016/j.aquaculture.2018.07.008",
language = "English",
volume = "496",
pages = "30--38",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",

}

Oocyte and egg quality indicators in European eel: Lipid droplet coalescence and fatty acid composition. / da Silva, Filipa ; Jacobsen, Charlotte; Kjørsvik, Elin; Støttrup, Josianne Gatt; Tomkiewicz, Jonna.

In: Aquaculture, Vol. 496, 2018, p. 30-38.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Oocyte and egg quality indicators in European eel: Lipid droplet coalescence and fatty acid composition

AU - da Silva, Filipa

AU - Jacobsen, Charlotte

AU - Kjørsvik, Elin

AU - Støttrup, Josianne Gatt

AU - Tomkiewicz, Jonna

PY - 2018

Y1 - 2018

N2 - During European eel assisted reproduction, timely administration of hormones that induce oocyte maturation and ovulation is a major factor influencing subsequent egg quality. This treatment commonly comprises one injection of fish pituitary extract (PE) as a primer followed by a 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) injection. In this context, the present study aimed at optimizing timing of the dual hormone administration by applying a lipid droplet-based oocyte maturation scale, previously developed for Japanese eel to determine the maturational status of each female. Using wild-caught female eels, the potential effect of female size, egg fatty acid composition and dry weight on egg quality was also analyzed. Larval survival at 3 days post hatch was used to differentiate High- and Low-quality egg batches. Results showed that lipid droplet diameter was significantly smaller in High-quality eggs than in Low-quality egg batches, indicating that females producing High-quality eggs received the PE primer and DHP generally at an earlier developmental stage than those producing Low-quality batches. These results confirm that oocyte lipid droplet diameter is a useful indicator of female maturational status for optimization of induction of oocyte maturation and ovulation in European eel. Additional parameters, including female size, egg fatty acid composition and dry weight, were similar between high and low quality egg batches. This insight regarding the fatty acid composition of eggs obtained from wild-caught female eels may help advancing the development of tailored diets for increased reproductive success of farmed broodstock

AB - During European eel assisted reproduction, timely administration of hormones that induce oocyte maturation and ovulation is a major factor influencing subsequent egg quality. This treatment commonly comprises one injection of fish pituitary extract (PE) as a primer followed by a 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) injection. In this context, the present study aimed at optimizing timing of the dual hormone administration by applying a lipid droplet-based oocyte maturation scale, previously developed for Japanese eel to determine the maturational status of each female. Using wild-caught female eels, the potential effect of female size, egg fatty acid composition and dry weight on egg quality was also analyzed. Larval survival at 3 days post hatch was used to differentiate High- and Low-quality egg batches. Results showed that lipid droplet diameter was significantly smaller in High-quality eggs than in Low-quality egg batches, indicating that females producing High-quality eggs received the PE primer and DHP generally at an earlier developmental stage than those producing Low-quality batches. These results confirm that oocyte lipid droplet diameter is a useful indicator of female maturational status for optimization of induction of oocyte maturation and ovulation in European eel. Additional parameters, including female size, egg fatty acid composition and dry weight, were similar between high and low quality egg batches. This insight regarding the fatty acid composition of eggs obtained from wild-caught female eels may help advancing the development of tailored diets for increased reproductive success of farmed broodstock

U2 - 10.1016/j.aquaculture.2018.07.008

DO - 10.1016/j.aquaculture.2018.07.008

M3 - Journal article

VL - 496

SP - 30

EP - 38

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -