One-Stage Tissue Engineering of Bladder Wall Patches for an Easy-To-Use Approach at the Surgical Table

Fatemeh Ajalloueian, Said Zeiai, Ramiro Rojas, Magdalena Fossum, Jons Hilborn

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We present a method for producing a cell-scaffold hybrid construct at the bedside. The construct is composed of plastic-compressed collagen together with a poly(e-caprolactone) (PCL)-knitted mesh that yields an integrated, natural-synthetic scaffold. This construct was evaluated by seeding of minced bladder mucosa, followed by proliferation in vitro. High mechanical strength in combination with a biological environment suitable for tissue growth was achieved through the creation of a hybrid construct that showed an increased tensile strength (17.9 +/- 2.6 MPa) when compared to plastic-compressed collagen (0.6 +/- 0.12 MPa). Intimate contact between the collagen and the PCL fabric was required to ensure integrity without delamination of the construct. This contact was achieved by surface alkaline hydrolysis of the PCL, followed by adsorption of poly(vinyl) alcohol. The improvement in hydrophilicity of the PCL-knitted mesh was confirmed through water contact angle measurements, and penetration of the collagen into the mesh was evaluated by scanning electron microscopy (SEM). Particles of minced bladder mucosa tissue were seeded onto this scaffold, and the proliferation was followed for 6 weeks in vitro. Results obtained from phase contrast microscopy, SEM, and histological staining indicated that cells migrated from the minced tissue particles and reorganized on the scaffold. Cells were viable and proliferative, with morphological features characteristic of urothelial cells. Proliferation reached the point at which a multilayer with a resemblance to stratified urothelium was achieved. This successful method could potentially be used for in vivo applications in reconstructive urology as an engineered autologous tissue transplant without the requirement for in vitro culture before transplantation.
Original languageEnglish
JournalTissue Engineering. Part C. Methods
Volume19
Issue number9
Pages (from-to)688-696
ISSN1937-3384
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)
  • Alkaline hydrolysis
  • Autologous tissue
  • Biological environments
  • High mechanical strength
  • Hybrid constructs
  • Morphological features
  • Phase-contrast microscopy
  • Water contact angle measurement
  • Cells
  • Collagen
  • Cytology
  • Scanning electron microscopy
  • Tensile strength
  • Tissue
  • Scaffolds (biology)
  • Animals
  • Compressive Strength
  • Microscopy, Electron, Scanning
  • Plastics
  • Polyesters
  • Rats
  • Surface Properties
  • Surgical Mesh
  • Tensile Strength
  • Textiles
  • Tissue Engineering
  • Tissue Scaffolds
  • Urinary Bladder
  • 24980-41-4 polycaprolactone
  • 9007-34-5 Collagen
  • CELL
  • BIOTECHNOLOGY
  • PLASTIC COMPRESSION
  • AUGMENTATION CYSTOPLASTY
  • BIOMECHANICAL PROPERTIES
  • EXTRACELLULAR-MATRIX
  • UROTHELIAL CELLS
  • COLLAGEN
  • REGENERATION
  • SCAFFOLDS
  • SKIN
  • SUBSTITUTES
  • biological environment
  • cell-scaffold hybrid construct
  • mesh tensile strength
  • morphological feature
  • natural-synthetic scaffold
  • plastic-compressed collagen polymer
  • poly(epsilon-caprolactone) knitted mesh
  • reconstructive urology
  • Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Suidae [85740] pig common
  • poly(vinyl) alcohol 9002-89-5
  • 02506, Cytology - Animal
  • 10511, Biophysics - Bioengineering
  • 11105, Anatomy and Histology - Surgery
  • 12512, Pathology - Therapy
  • 15504, Urinary system - Physiology and biochemistry
  • 32500, Tissue culture, apparatus, methods and media
  • Chemical Coordination and Homeostasis
  • Medical Sciences
  • bladder mucosa excretory system
  • bladder wall patches excretory system
  • urothelial cell excretory system
  • histological staining laboratory techniques, histology and cytology techniques
  • in vitro culture laboratory techniques, culturing techniques
  • one-stage tissue engineering laboratory techniques, culturing techniques
  • scanning electron microscopy SEM laboratory techniques, imaging and microscopy techniques
  • surface alkaline hydrolysis laboratory techniques
  • surgical table laboratory equipment
  • transplantation therapy therapeutic and prophylactic techniques, clinical techniques
  • water contact angle measurement laboratory techniques
  • Biomaterials
  • Methods and Techniques
  • Surgery
  • Urinary System

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