On the mechanism of oscillations in neutrophils

Jens Christian Brasen, Torben Barington, Lars Folke Olsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have investigated the regulation of the oscillatory generation of H2O2 and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H2O2 production are 180 degrees out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H2O2 production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H2O2 production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations. (C) 2010 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalBiophysical Chemistry
Volume148
Issue number1-3
Pages (from-to)82-92
ISSN0301-4622
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Biochemistry
  • Biophysics
  • Organic Chemistry
  • NAD(P)H
  • NADPH oxidase
  • Neutrophil
  • Oscillations
  • Protein kinase C
  • 2 butanol
  • actin
  • butanol
  • cytochalasin B
  • formylmethionylleucylphenylalanine
  • glucose
  • hydrogen peroxide
  • phorbol 13 acetate 12 myristate
  • phosphatidylinositol 3 kinase
  • reduced nicotinamide adenine dinucleotide phosphate
  • article
  • cell density
  • cell motion
  • cell shape
  • cell size
  • concentration (parameters)
  • controlled study
  • feedback system
  • fluorescence analysis
  • human
  • human cell
  • mathematical model
  • neutrophil
  • normal human
  • oscillation
  • priority journal
  • protein polymerization
  • qualitative analysis
  • signal transduction
  • 1-Butanol
  • 1-Phosphatidylinositol 3-Kinase
  • Calcium
  • Cell Count
  • Cytochalasin B
  • Feedback, Physiological
  • Humans
  • Hydrogen Peroxide
  • Models, Biological
  • NADP
  • Neutrophils
  • Phospholipase D
  • Protein Multimerization
  • Proto-Oncogene Proteins c-akt
  • Phosphatidylinositol 3-Kinases
  • 3CHI920QS7 Cytochalasin B
  • 53-59-8 NADP
  • 8PJ61P6TS3 1-Butanol
  • BBX060AN9V Hydrogen Peroxide
  • EC 2.7.1.- Phosphatidylinositol 3-Kinases
  • EC 2.7.11.1 Proto-Oncogene Proteins c-akt
  • EC 3.1.4.4 Phospholipase D
  • SY7Q814VUP Calcium
  • BIOCHEMISTRY
  • BIOPHYSICS
  • CHEMISTRY,
  • HUMAN POLYMORPHONUCLEAR LEUKOCYTES
  • PEROXIDASE-OXIDASE REACTION
  • PROTEIN-KINASE-C
  • NADPH-OXIDASE
  • SACCHAROMYCES-CEREVISIAE
  • PHOSPHOLIPASE-D
  • MEMBRANE
  • CELL
  • ACTIVATION
  • DYNAMICS
  • cell oscillation mechanism
  • feedback regulation
  • signal transduction cascade
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common
  • 1-butanol 71-36-3
  • 2-butanol 78-92-2
  • cytochalasin 37187-49-8
  • glucose 58367-01-4
  • hydrogen peroxide 7722-84-1
  • NADPH 53-57-6
  • phosphoinositide 3-kinase PI3K 115926-52-8 EC 2.7.1.154
  • Rac
  • 02506, Cytology - Animal
  • 02508, Cytology - Human
  • 04500, Mathematical biology and statistical methods
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10068, Biochemistry studies - Carbohydrates
  • 10515, Biophysics - Biocybernetics
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 34502, Immunology - General and methods
  • Chemical Coordination and Homeostasis
  • Computational Biology
  • neutrophil immune system, blood and lymphatics
  • mathematical model mathematical and computer techniques
  • mechanistic model mathematical and computer techniques
  • Biochemistry and Molecular Biophysics
  • Immune System
  • Models and Simulations

Cite this

Brasen, J. C., Barington, T., & Olsen, L. F. (2010). On the mechanism of oscillations in neutrophils. Biophysical Chemistry, 148(1-3), 82-92. https://doi.org/10.1016/j.bpc.2010.02.013