On-line monitoring of Glucose and penicillin by sequential injection analysis

R.W. Min, Jens Bredal Nielsen, John Villadsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A sequential injection analysis (SIA) system has been developed for on-line monitoring of glucose and penicillin during cultivations of the filamentous fungus Penicillium chrysogenum. The SIA system consists of a peristaltic pump, an injection valve, two piston pumps, two multi-position valves and a detector. The glucose analyzer is based on an enzymatic reaction using glucose oxidase, which converts glucose to glucono-lactone with formation of hydrogen peroxide and subsequent detection of H2O2 by a chemiluminescence reaction involving luminol. The penicillin analysis is based on formation of penicilloic acid by penicillinase. Penicilloic acid is detected either by a chemiluminescence method or by a decolorization method. In the chemiluminescence method the penicilloic acid is quantified by its quenching effect on the chemiluminescence signal obtained when luminol reacts with iodine. In the decolorization method the penicilloic acid is detected spectrophotometrically by the decrease in the absorbance of an iodine-starch complex.
    Original languageEnglish
    JournalAnalytica Chimica Acta
    Volume320
    Issue number2-3
    Pages (from-to)199-205
    ISSN0030-2670
    DOIs
    Publication statusPublished - 1996

    Fingerprint

    Dive into the research topics of 'On-line monitoring of Glucose and penicillin by sequential injection analysis'. Together they form a unique fingerprint.

    Cite this