On-line monitoring of fermentation processes using multi-wavelength fluorescence

Peter Odman, Nanna Petersen, Claus Lindvald Johansen, Lisbeth Olsson, Krist Gernaey, Anna Eliasson Lantz

Research output: Contribution to journalConference abstract in journalResearchpeer-review

Abstract

Fermentation processes often suffer from a lack of real-time methods for on-line determination of variables like the concentrations of nutrients and products. This work aims at investigating the possibilities of implementing an on-line fermentation monitoring system based on multi-wavelength fluorescence (MWF). This type of sensor has previously showed promising accuracy and selectivity for in situ monitoring of cell mass and certain metabolites in bioreactors (Lantz et al., 2006). The sensor generates multivariate data outputs, which necessitate chemometric modeling for signal interpretation.

The model system considered in this work is the antibiotic production by Streptomyces coelicolor, a filamentous bacterium. In addition to predicting concentrations of biomass in the fermentation broth, the data allowed detection of different physiological states, i.e. growth phase and phosphate limitation. The data was analyzed using Parallel Factor Analysis (PARAFAC), which may allow the identification of individual analytes in the mixture from their specific 3-way profile (Bro, 1997).
Original languageEnglish
JournalJournal of Biotechnology
Volume131
Issue number2, Supplement
Pages (from-to)187-187
ISSN0168-1656
DOIs
Publication statusPublished - 2007
Event13th European Congress on Biotechnology - Barcelona, Spain
Duration: 17 Sep 200719 Sep 2007
Conference number: 13

Conference

Conference13th European Congress on Biotechnology
Number13
CountrySpain
CityBarcelona
Period17/09/200719/09/2007

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