Abstract
Fermentation processes often suffer from a lack of real-time methods for on-line determination of variables like the concentrations of nutrients and products. This work aims at investigating the possibilities of implementing an on-line fermentation monitoring system based on multi-wavelength fluorescence (MWF). This type of sensor has previously showed promising accuracy and selectivity for in situ monitoring of cell mass and certain metabolites in bioreactors (Lantz et al., 2006). The sensor generates multivariate data outputs, which necessitate chemometric modeling for signal interpretation.
The model system considered in this work is the antibiotic production by Streptomyces coelicolor, a filamentous bacterium. In addition to predicting concentrations of biomass in the fermentation broth, the data allowed detection of different physiological states, i.e. growth phase and phosphate limitation. The data was analyzed using Parallel Factor Analysis (PARAFAC), which may allow the identification of individual analytes in the mixture from their specific 3-way profile (Bro, 1997).
The model system considered in this work is the antibiotic production by Streptomyces coelicolor, a filamentous bacterium. In addition to predicting concentrations of biomass in the fermentation broth, the data allowed detection of different physiological states, i.e. growth phase and phosphate limitation. The data was analyzed using Parallel Factor Analysis (PARAFAC), which may allow the identification of individual analytes in the mixture from their specific 3-way profile (Bro, 1997).
Original language | English |
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Journal | Journal of Biotechnology |
Volume | 131 |
Issue number | 2, Supplement |
Pages (from-to) | 187-187 |
ISSN | 0168-1656 |
DOIs | |
Publication status | Published - 2007 |
Event | 13th European Congress on Biotechnology - Barcelona, Spain Duration: 17 Sept 2007 → 19 Sept 2007 Conference number: 13 |
Conference
Conference | 13th European Congress on Biotechnology |
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Number | 13 |
Country/Territory | Spain |
City | Barcelona |
Period | 17/09/2007 → 19/09/2007 |