On-line measurements of oscillating mitochondrial membrane potential in glucose-fermenting Saccharomyces cerevisiae

Ann Zahle Andersen, Allan K. Poulsen, Jens Christian Brasen, Lars Folke Olsen

Research output: Contribution to journalJournal articleResearchpeer-review


We employed the fluorescent cyanine dye DiOC(2)(3) to measure membrane potential in semi-anaerobic yeast cells under conditions where glycolysis was oscillating. Oscillations in glycolysis were studied by means of the naturally abundant nicotinamide adenine dinucleotide (NADH). We found that the mitochondrial membrane potential was oscillating, and that these oscillations displayed the same frequency and duration as the NADH oscillations. It was confirmed that DiOC(2)(3) localizes itself in the mitochondrial membrane and thus reports qualitative changes solely in mitochondrial membrane potential. Our studies showed that glycolytic oscillations perturb the mitochondrial membrane potential and that the mitochondria do not have any controlling effect on the dynamics of glycolysis under these conditions. Depolarization of the mitochondrial membrane by addition of FCCP quenched mitochondrial membrane potential oscillations and delocalized DiOC(2)(3), while glycolysis continued to oscillate unaffected. Copyright (c) 2007 John Wiley & Sons, Ltd.
Original languageEnglish
Issue number9
Pages (from-to)731-739
Publication statusPublished - 2007
Externally publishedYes


  • Biological Clocks
  • Carbocyanines
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
  • Fluorescent Dyes
  • Glycolysis
  • Membrane Potential, Mitochondrial
  • Microscopy, Fluorescence
  • Microscopy, Phase-Contrast
  • NAD
  • Saccharomyces cerevisiae
  • Uncoupling Agents
  • 0U46U6E8UK NAD
  • 370-86-5 Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
  • 37069-75-3 3,3'-diethyloxacarbocyanine
  • glycolysis
  • membrane potential
  • on-line measurement
  • oscillation
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Saccharomyces cerevisiae species
  • cyanine dye
  • glucose 58367-01-4
  • NAD 53-84-9
  • 02504, Cytology - Plant
  • 03502, Genetics - General
  • 03504, Genetics - Plant
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10068, Biochemistry studies - Carbohydrates
  • 10508, Biophysics - Membrane phenomena
  • Biochemistry and Molecular Biophysics
  • Cell Biology
  • mitochondria
  • mitochondrial membrane
  • Membranes
  • Molecular Genetics


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