On-Chip integration of sample pretreatment and Multiplex polymerase chain reaction (PCR) for DNA analysis

Monica Brivio, Detlef Snakenborg, E. Søgaard, A. Ahlford, A.-C Syvänen, Jörg Peter Kutter, Anders Wolff

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    Abstract

    In this paper we present a modular lab-on-a-chip system for integrated sample
    pre-treatment (PT) by magnetophoresis and DNA amplification by polymerase chain
    reaction (PCR). It consists of a polymer-based microfluidic chip mounted on a custom-made thermocycler (Figure 1) and includes a simple and efficient method for
    switching the liquid flow between the PT and PCR chamber. Purification of human
    genomic DNA from EDTA-treated blood and multiplex PCR were successfully carried
    out on-chip using the developed lab-on-a-chip system.
    Original languageEnglish
    Title of host publicationProceeding of "uTas"
    PublisherChemical and Biological Microsystems Society
    Publication date2008
    Pages1737-1739
    ISBN (Print)978-0-9798064-1-4
    Publication statusPublished - 2008
    Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - San Diego Sheraton Hotel & Marina, San Diego, United States
    Duration: 12 Oct 200816 Oct 2008
    Conference number: 12

    Conference

    Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Number12
    LocationSan Diego Sheraton Hotel & Marina
    CountryUnited States
    CitySan Diego
    Period12/10/200816/10/2008

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