On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae

Frederik Westergaard Østerberg, Giovanni Rizzi, Marco Donolato, R.S. Bejhed, A. Mezger, M. Strömberg, M. Nilsson, M. Strømme, P. Svedlindh, Mikkel Fougt Hansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    For the first time DNA coils formed by rolling circle amplification are quantified
    on-chip by Brownian relaxation measurements on magnetic nanobeads using a
    magnetoresistive sensor. No external magnetic fields are required besides the magnetic field arising from the current through the sensor, which makes the setup very compact. Limits of detection down to 500 Bacillus globigii spores and 2 pM of Vibrio cholerae are demonstrated, which are on the same order of magnitude or lower than those achieved previously using a commercial macro-scale AC susceptometer. The chipbased readout is an important step towards the realization of field tests based on rolling circle amplification molecular analyses.
    Original languageEnglish
    JournalSmall
    Volume10
    Issue number14
    Pages (from-to)2877-2882
    Number of pages6
    ISSN1613-6810
    DOIs
    Publication statusPublished - 2014

    Cite this

    Østerberg, F. W., Rizzi, G., Donolato, M., Bejhed, R. S., Mezger, A., Strömberg, M., ... Hansen, M. F. (2014). On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae. Small, 10(14), 2877-2882. https://doi.org/10.1002/smll.201303325
    Østerberg, Frederik Westergaard ; Rizzi, Giovanni ; Donolato, Marco ; Bejhed, R.S. ; Mezger, A. ; Strömberg, M. ; Nilsson, M. ; Strømme, M. ; Svedlindh, P. ; Hansen, Mikkel Fougt. / On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae. In: Small. 2014 ; Vol. 10, No. 14. pp. 2877-2882.
    @article{e4971fa3007946c197c84978aa2a00fd,
    title = "On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae",
    abstract = "For the first time DNA coils formed by rolling circle amplification are quantifiedon-chip by Brownian relaxation measurements on magnetic nanobeads using amagnetoresistive sensor. No external magnetic fields are required besides the magnetic field arising from the current through the sensor, which makes the setup very compact. Limits of detection down to 500 Bacillus globigii spores and 2 pM of Vibrio cholerae are demonstrated, which are on the same order of magnitude or lower than those achieved previously using a commercial macro-scale AC susceptometer. The chipbased readout is an important step towards the realization of field tests based on rolling circle amplification molecular analyses.",
    author = "{\O}sterberg, {Frederik Westergaard} and Giovanni Rizzi and Marco Donolato and R.S. Bejhed and A. Mezger and M. Str{\"o}mberg and M. Nilsson and M. Str{\o}mme and P. Svedlindh and Hansen, {Mikkel Fougt}",
    year = "2014",
    doi = "10.1002/smll.201303325",
    language = "English",
    volume = "10",
    pages = "2877--2882",
    journal = "Small",
    issn = "1613-6810",
    publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
    number = "14",

    }

    Østerberg, FW, Rizzi, G, Donolato, M, Bejhed, RS, Mezger, A, Strömberg, M, Nilsson, M, Strømme, M, Svedlindh, P & Hansen, MF 2014, 'On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae', Small, vol. 10, no. 14, pp. 2877-2882. https://doi.org/10.1002/smll.201303325

    On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae. / Østerberg, Frederik Westergaard; Rizzi, Giovanni; Donolato, Marco; Bejhed, R.S.; Mezger, A.; Strömberg, M.; Nilsson, M.; Strømme, M.; Svedlindh, P.; Hansen, Mikkel Fougt.

    In: Small, Vol. 10, No. 14, 2014, p. 2877-2882.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae

    AU - Østerberg, Frederik Westergaard

    AU - Rizzi, Giovanni

    AU - Donolato, Marco

    AU - Bejhed, R.S.

    AU - Mezger, A.

    AU - Strömberg, M.

    AU - Nilsson, M.

    AU - Strømme, M.

    AU - Svedlindh, P.

    AU - Hansen, Mikkel Fougt

    PY - 2014

    Y1 - 2014

    N2 - For the first time DNA coils formed by rolling circle amplification are quantifiedon-chip by Brownian relaxation measurements on magnetic nanobeads using amagnetoresistive sensor. No external magnetic fields are required besides the magnetic field arising from the current through the sensor, which makes the setup very compact. Limits of detection down to 500 Bacillus globigii spores and 2 pM of Vibrio cholerae are demonstrated, which are on the same order of magnitude or lower than those achieved previously using a commercial macro-scale AC susceptometer. The chipbased readout is an important step towards the realization of field tests based on rolling circle amplification molecular analyses.

    AB - For the first time DNA coils formed by rolling circle amplification are quantifiedon-chip by Brownian relaxation measurements on magnetic nanobeads using amagnetoresistive sensor. No external magnetic fields are required besides the magnetic field arising from the current through the sensor, which makes the setup very compact. Limits of detection down to 500 Bacillus globigii spores and 2 pM of Vibrio cholerae are demonstrated, which are on the same order of magnitude or lower than those achieved previously using a commercial macro-scale AC susceptometer. The chipbased readout is an important step towards the realization of field tests based on rolling circle amplification molecular analyses.

    U2 - 10.1002/smll.201303325

    DO - 10.1002/smll.201303325

    M3 - Journal article

    VL - 10

    SP - 2877

    EP - 2882

    JO - Small

    JF - Small

    SN - 1613-6810

    IS - 14

    ER -

    Østerberg FW, Rizzi G, Donolato M, Bejhed RS, Mezger A, Strömberg M et al. On-chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii spores and Vibrio Cholerae. Small. 2014;10(14):2877-2882. https://doi.org/10.1002/smll.201303325