Oil adjuvant elevates protection of rainbow trout (Oncorhynchus my-kiss) following injection vaccination against Yersinia ruckeri

Rzgar M. Jaafar, Jiwan Kumar Chettri, Inger Dalsgaard, Azmi Al-Jubury, Per W. Kania, Jakob Skov, Kurt Buchmann

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

    Abstract

    Enteric redmouth disease (ERM) caused by the fish pathogen Yersinia ruckeri is a major threat to freshwater production of rainbow trout throughout all life stages. Injection vaccination of rainbow trout against Y. ruckeri infection has been shown to confer better protection compared to the traditionally applied immersion vaccination. It may be hypothesized, based on experience from other vaccines, that adjuvants may increase the protective level of ERM injection vaccines even more. Controlled comparative vaccination studies conducted to investigate effects of the oil adjuvant Montanide™ ISA 763 A VG when added to an experimental Y. ruckeri bacterin (containing both biotype 1 and 2 of serotype O1). A total of 1000 fish with mean weight 19 g was divided into five different groups (in duplicated tanks 2 • 100 fish per group) 1) non-vaccinated control fish (NonVac), 2) fish injected with a commercial vaccine (AquaVac® Relera™) (ComVac), 3) fish injected with an experimental vaccine (ExpVac), 4) fish injected with an experimental vaccine + adjuvant (ExpVacAdj) and 5) fish injected with adjuvant alone (Adj). Injection of the experimental vaccine (both adjuvanted and non-adjuvanted) induced a significantly higher antibody (IgM) level, increased occurrence of IgM+ cells in spleen tissue and significant up-regulation of several immune genes. Additional experiments using a higher challenge dosage suggested an immune enhancing effect of the adjuvant as the challenge produced 100% mortality in the NonVac group, 60% mortality in both of ComVac and Adj groups and only 13 and 2.5% mortalities in the ExpVac and the ExpVacAdj groups, respectively.
    Original languageEnglish
    Article numberO-022
    JournalFish and Shellfish Immunology
    Volume53
    Pages (from-to)65-66
    Number of pages2
    ISSN1050-4648
    DOIs
    Publication statusPublished - 2016
    Event2nd International Conference of Fish & Shellfish Immunology: ISFSI 2016 - Maine, United States
    Duration: 26 Jun 201630 Jun 2016
    Conference number: 2

    Conference

    Conference2nd International Conference of Fish & Shellfish Immunology
    Number2
    CountryUnited States
    CityMaine
    Period26/06/201630/06/2016

    Keywords

    • Rainbow trout
    • Yersinia ruckeri
    • Vaccination
    • Adjuvant

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