Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market

Aline M. Katsurayama, Ligia Manoel Martins, Beatriz T. Iamanaka, Maria Helena P. Fungaro, Josué J da Silva, Jens Christian Frisvad, John I. Pitt, Marta H. Taniwaki*

*Corresponding author for this work

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Abstract

The guarantee of the high quality of rice is of utmost importance because any toxic contaminant may affect consumer health, especially in countries such as Brazil where rice is part of the daily diet. A total of 187 rice samples, from field, processing and market from two different production systems, wetland from the state of Rio Grande do Sul, dryland, from the state of Maranhão and market samples from the state of São Paulo, were analyzed for fungi belonging to Aspergillus section Flavi and the presence of aflatoxins. Twenty-three soil samples from wetland and dryland were also analyzed. A total of 383 Aspergillus section Flavi strains were isolated from rice and soil samples. Using a polyphasic approach, with phenotypic (morphology and extrolite profiles) and molecular data (beta-tubulin gene sequences), five species were identified: A. flavus, A. caelatus, A. novoparasiticus, A. arachidicola and A. pseudocaelatus. This is the first report of these last three species from rice and rice plantation soil. Only seven (17%) of the A. flavus isolates produced type B aflatoxins, but 95% produced kojic acid and 69% cyclopiazonic acid. Less than 14% of the rice samples were contaminated with aflatoxins, but two of the market samples were well above the maximum tolerable limit (5 μg/kg), established by the Brazilian National Health Surveillance Agency.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume266
Pages (from-to)213-221
Number of pages31
ISSN0168-1605
DOIs
Publication statusPublished - 2018

Cite this

Katsurayama, A. M., Martins, L. M., Iamanaka, B. T., Fungaro, M. H. P., da Silva, J. J., Frisvad, J. C., ... Taniwaki, M. H. (2018). Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market. International Journal of Food Microbiology, 266, 213-221. https://doi.org/10.1016/j.ijfoodmicro.2017.12.008
Katsurayama, Aline M. ; Martins, Ligia Manoel ; Iamanaka, Beatriz T. ; Fungaro, Maria Helena P. ; da Silva, Josué J ; Frisvad, Jens Christian ; Pitt, John I. ; Taniwaki, Marta H. / Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market. In: International Journal of Food Microbiology. 2018 ; Vol. 266. pp. 213-221.
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title = "Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market",
abstract = "The guarantee of the high quality of rice is of utmost importance because any toxic contaminant may affect consumer health, especially in countries such as Brazil where rice is part of the daily diet. A total of 187 rice samples, from field, processing and market from two different production systems, wetland from the state of Rio Grande do Sul, dryland, from the state of Maranh{\~a}o and market samples from the state of S{\~a}o Paulo, were analyzed for fungi belonging to Aspergillus section Flavi and the presence of aflatoxins. Twenty-three soil samples from wetland and dryland were also analyzed. A total of 383 Aspergillus section Flavi strains were isolated from rice and soil samples. Using a polyphasic approach, with phenotypic (morphology and extrolite profiles) and molecular data (beta-tubulin gene sequences), five species were identified: A. flavus, A. caelatus, A. novoparasiticus, A. arachidicola and A. pseudocaelatus. This is the first report of these last three species from rice and rice plantation soil. Only seven (17{\%}) of the A. flavus isolates produced type B aflatoxins, but 95{\%} produced kojic acid and 69{\%} cyclopiazonic acid. Less than 14{\%} of the rice samples were contaminated with aflatoxins, but two of the market samples were well above the maximum tolerable limit (5 μg/kg), established by the Brazilian National Health Surveillance Agency.",
author = "Katsurayama, {Aline M.} and Martins, {Ligia Manoel} and Iamanaka, {Beatriz T.} and Fungaro, {Maria Helena P.} and {da Silva}, {Josu{\'e} J} and Frisvad, {Jens Christian} and Pitt, {John I.} and Taniwaki, {Marta H.}",
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volume = "266",
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journal = "International Journal of Food Microbiology",
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Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market. / Katsurayama, Aline M.; Martins, Ligia Manoel; Iamanaka, Beatriz T.; Fungaro, Maria Helena P.; da Silva, Josué J; Frisvad, Jens Christian; Pitt, John I.; Taniwaki, Marta H.

In: International Journal of Food Microbiology, Vol. 266, 2018, p. 213-221.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Occurrence of Aspergillus section Flavi and aflatoxins in Brazilian rice: From field to market

AU - Katsurayama, Aline M.

AU - Martins, Ligia Manoel

AU - Iamanaka, Beatriz T.

AU - Fungaro, Maria Helena P.

AU - da Silva, Josué J

AU - Frisvad, Jens Christian

AU - Pitt, John I.

AU - Taniwaki, Marta H.

PY - 2018

Y1 - 2018

N2 - The guarantee of the high quality of rice is of utmost importance because any toxic contaminant may affect consumer health, especially in countries such as Brazil where rice is part of the daily diet. A total of 187 rice samples, from field, processing and market from two different production systems, wetland from the state of Rio Grande do Sul, dryland, from the state of Maranhão and market samples from the state of São Paulo, were analyzed for fungi belonging to Aspergillus section Flavi and the presence of aflatoxins. Twenty-three soil samples from wetland and dryland were also analyzed. A total of 383 Aspergillus section Flavi strains were isolated from rice and soil samples. Using a polyphasic approach, with phenotypic (morphology and extrolite profiles) and molecular data (beta-tubulin gene sequences), five species were identified: A. flavus, A. caelatus, A. novoparasiticus, A. arachidicola and A. pseudocaelatus. This is the first report of these last three species from rice and rice plantation soil. Only seven (17%) of the A. flavus isolates produced type B aflatoxins, but 95% produced kojic acid and 69% cyclopiazonic acid. Less than 14% of the rice samples were contaminated with aflatoxins, but two of the market samples were well above the maximum tolerable limit (5 μg/kg), established by the Brazilian National Health Surveillance Agency.

AB - The guarantee of the high quality of rice is of utmost importance because any toxic contaminant may affect consumer health, especially in countries such as Brazil where rice is part of the daily diet. A total of 187 rice samples, from field, processing and market from two different production systems, wetland from the state of Rio Grande do Sul, dryland, from the state of Maranhão and market samples from the state of São Paulo, were analyzed for fungi belonging to Aspergillus section Flavi and the presence of aflatoxins. Twenty-three soil samples from wetland and dryland were also analyzed. A total of 383 Aspergillus section Flavi strains were isolated from rice and soil samples. Using a polyphasic approach, with phenotypic (morphology and extrolite profiles) and molecular data (beta-tubulin gene sequences), five species were identified: A. flavus, A. caelatus, A. novoparasiticus, A. arachidicola and A. pseudocaelatus. This is the first report of these last three species from rice and rice plantation soil. Only seven (17%) of the A. flavus isolates produced type B aflatoxins, but 95% produced kojic acid and 69% cyclopiazonic acid. Less than 14% of the rice samples were contaminated with aflatoxins, but two of the market samples were well above the maximum tolerable limit (5 μg/kg), established by the Brazilian National Health Surveillance Agency.

U2 - 10.1016/j.ijfoodmicro.2017.12.008

DO - 10.1016/j.ijfoodmicro.2017.12.008

M3 - Journal article

VL - 266

SP - 213

EP - 221

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

ER -