Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Liese Van Gompel*, Wietske Dohmen, Roosmarijn E. C. Luiken, Martijn Bouwknegt, Lourens Heres, Eri Van Heijnsbergen, Betty G M Jongerius-Gortemaker, Peter Scherpenisse, Gerdit D Greve, Monique H G Tersteeg-Zijderveld, Katharina Wadepohl, Ana Sofia Ribeiro Duarte, Violeta Muñoz-Gómez, Jennie Fischer, Magdalena Skarżyńska, Dariusz Wasyl, Jaap A Wagenaar, Bert A P Urlings, Alejandro Dorado-García, Inge M WoutersDick J J Heederik, Heike Schmitt, Lidwien A M Smit

*Corresponding author for this work

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Abstract

Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. During 2015-2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW, ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage. Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW, ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage. We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage.
Original languageEnglish
Article numberwxz098
JournalAnnals of Work Exposures and Health
Pages (from-to)1-13
ISSN2398-7308
DOIs
Publication statusAccepted/In press - 2020

Cite this

Van Gompel, L., Dohmen, W., Luiken, R. E. C., Bouwknegt, M., Heres, L., Van Heijnsbergen, E., ... Smit, L. A. M. (Accepted/In press). Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers. Annals of Work Exposures and Health, 1-13. [wxz098]. https://doi.org/10.1093/annweh/wxz098
Van Gompel, Liese ; Dohmen, Wietske ; Luiken, Roosmarijn E. C. ; Bouwknegt, Martijn ; Heres, Lourens ; Van Heijnsbergen, Eri ; Jongerius-Gortemaker, Betty G M ; Scherpenisse, Peter ; Greve, Gerdit D ; Tersteeg-Zijderveld, Monique H G ; Wadepohl, Katharina ; Ribeiro Duarte, Ana Sofia ; Muñoz-Gómez, Violeta ; Fischer, Jennie ; Skarżyńska, Magdalena ; Wasyl, Dariusz ; Wagenaar, Jaap A ; Urlings, Bert A P ; Dorado-García, Alejandro ; Wouters, Inge M ; Heederik, Dick J J ; Schmitt, Heike ; Smit, Lidwien A M. / Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers. In: Annals of Work Exposures and Health. 2020 ; pp. 1-13.
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title = "Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers",
abstract = "Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. During 2015-2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW, ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage. Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW, ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage. We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage.",
author = "{Van Gompel}, Liese and Wietske Dohmen and Luiken, {Roosmarijn E. C.} and Martijn Bouwknegt and Lourens Heres and {Van Heijnsbergen}, Eri and Jongerius-Gortemaker, {Betty G M} and Peter Scherpenisse and Greve, {Gerdit D} and Tersteeg-Zijderveld, {Monique H G} and Katharina Wadepohl and {Ribeiro Duarte}, {Ana Sofia} and Violeta Mu{\~n}oz-G{\'o}mez and Jennie Fischer and Magdalena Skarżyńska and Dariusz Wasyl and Wagenaar, {Jaap A} and Urlings, {Bert A P} and Alejandro Dorado-Garc{\'i}a and Wouters, {Inge M} and Heederik, {Dick J J} and Heike Schmitt and Smit, {Lidwien A M}",
year = "2020",
doi = "10.1093/annweh/wxz098",
language = "English",
pages = "1--13",
journal = "Annals of Work Exposures and Health",
issn = "2398-7308",
publisher = "Oxford University Press",

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Van Gompel, L, Dohmen, W, Luiken, REC, Bouwknegt, M, Heres, L, Van Heijnsbergen, E, Jongerius-Gortemaker, BGM, Scherpenisse, P, Greve, GD, Tersteeg-Zijderveld, MHG, Wadepohl, K, Ribeiro Duarte, AS, Muñoz-Gómez, V, Fischer, J, Skarżyńska, M, Wasyl, D, Wagenaar, JA, Urlings, BAP, Dorado-García, A, Wouters, IM, Heederik, DJJ, Schmitt, H & Smit, LAM 2020, 'Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers', Annals of Work Exposures and Health, pp. 1-13. https://doi.org/10.1093/annweh/wxz098

Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers. / Van Gompel, Liese; Dohmen, Wietske; Luiken, Roosmarijn E. C.; Bouwknegt, Martijn; Heres, Lourens; Van Heijnsbergen, Eri; Jongerius-Gortemaker, Betty G M; Scherpenisse, Peter; Greve, Gerdit D; Tersteeg-Zijderveld, Monique H G; Wadepohl, Katharina; Ribeiro Duarte, Ana Sofia; Muñoz-Gómez, Violeta; Fischer, Jennie; Skarżyńska, Magdalena; Wasyl, Dariusz; Wagenaar, Jaap A; Urlings, Bert A P; Dorado-García, Alejandro; Wouters, Inge M; Heederik, Dick J J; Schmitt, Heike; Smit, Lidwien A M.

In: Annals of Work Exposures and Health, 2020, p. 1-13.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

AU - Van Gompel, Liese

AU - Dohmen, Wietske

AU - Luiken, Roosmarijn E. C.

AU - Bouwknegt, Martijn

AU - Heres, Lourens

AU - Van Heijnsbergen, Eri

AU - Jongerius-Gortemaker, Betty G M

AU - Scherpenisse, Peter

AU - Greve, Gerdit D

AU - Tersteeg-Zijderveld, Monique H G

AU - Wadepohl, Katharina

AU - Ribeiro Duarte, Ana Sofia

AU - Muñoz-Gómez, Violeta

AU - Fischer, Jennie

AU - Skarżyńska, Magdalena

AU - Wasyl, Dariusz

AU - Wagenaar, Jaap A

AU - Urlings, Bert A P

AU - Dorado-García, Alejandro

AU - Wouters, Inge M

AU - Heederik, Dick J J

AU - Schmitt, Heike

AU - Smit, Lidwien A M

PY - 2020

Y1 - 2020

N2 - Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. During 2015-2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW, ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage. Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW, ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage. We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage.

AB - Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. During 2015-2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW, ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage. Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW, ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage. We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage.

U2 - 10.1093/annweh/wxz098

DO - 10.1093/annweh/wxz098

M3 - Journal article

C2 - 31883001

SP - 1

EP - 13

JO - Annals of Work Exposures and Health

JF - Annals of Work Exposures and Health

SN - 2398-7308

M1 - wxz098

ER -