Nucleic Acid Analogue Induced Transcription of Double Stranded DNA

Rolf Henrik Berg (Inventor), Ole Buchardt (Inventor), Michael Engholm (Inventor), Peter E. Nielsen (Inventor)

    Research output: Patent

    102 Downloads (Pure)


    RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.
    Original languageEnglish
    Patent numberUS5837459
    Filing date17/11/1998
    Publication statusPublished - 1998

    Bibliographical note

    Also published as: GB2284209 (A) GB2284209 (A8) JPH09503393 (A) JP3143475 (B2) ES2133709 (T3) EP0730664 (A1) EP0730664 (B1) WO9514789 (A1) DK0730664 (T3) DE69416692 (T2) CA2176746 (A1) CA2176746 (C) AT176932 (T)


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