Nucleic Acid Analogue Induced Transcription of Double Stranded DNA

Rolf Henrik Berg (Inventor), Ole Buchardt (Inventor), Michael Engholm (Inventor), Peter E. Nielsen (Inventor)

    Research output: Patent

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    Abstract

    RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.
    Original languageEnglish
    Patent numberUS5837459
    Filing date17/11/1998
    CountryDenmark
    Publication statusPublished - 1998

    Bibliographical note

    Also published as: GB2284209 (A) GB2284209 (A8) JPH09503393 (A) JP3143475 (B2) ES2133709 (T3) EP0730664 (A1) EP0730664 (B1) WO9514789 (A1) DK0730664 (T3) DE69416692 (T2) CA2176746 (A1) CA2176746 (C) AT176932 (T)

    Cite this

    Berg, R. H., Buchardt, O., Engholm, M., & Nielsen, P. E. (1998). Nucleic Acid Analogue Induced Transcription of Double Stranded DNA. (Patent No. US5837459).