Novel Vaccine Against Mycoplasma Hyosynoviae: The Immunogenic Effect of Iscom-Based Vaccines in Swine

Klara Tølbøll Lauritsen, Annette Vinther Heydenreich, Ulla Riber, Jeanne Toft Jakobsen, Nikolai Kirkby, Gregers Jungersen

    Research output: Contribution to conferenceConference abstract for conferenceResearch


    Arthritis in swine is frequently caused by Mycoplasma hyosynoviae (Mhs). For the development of an effective vaccine we investigated the immunogenic effect of three vaccine preparations with the ISCOM adjuvant Posintro™ from Nordic Vaccine. A: formalin fixed whole-cells Mhs (300 µg/dose) mixed with Posintro, B: Deoxycholate extracted lipoproteins from Mhs organisms (DOC-antigen, 300 μg/dose) in Posintro and C: DOC-antigen (50 μg/dose) in Posintro. Each vaccine-group contained three pigs. Vaccinations (i.m.) were performed at 12 and 15 weeks of age. The development of specific IgG and secretion of IFNγ were measured. Three weeks after the second vaccination, pigs were euthanised and autopsied. Vaccine B induced a high level of specific serum IgG in all pigs a week after boost. Vaccine C gave a variable response after boost, with two pigs seroconverting, while no response was seen by vaccine A. IFNγ in supernatants of whole-blood cultured with Mhs-antigen was used as a marker of cell-mediated immune response (CMI). All pigs secreted IFNγ after primary vaccination followed by an increased production after booster vaccination. The CMI response was highest with vaccine B when compared to responses induced by vaccine A and C at one (P
    Original languageEnglish
    Publication date2009
    Publication statusPublished - 2009
    Event3rd European Veterinary Immunology Workshop - Berlin, Germany
    Duration: 1 Sept 200913 Sept 2009
    Conference number: 3rd


    Conference3rd European Veterinary Immunology Workshop


    • Swine, vaccine, Mycoplasma hyosynoviae, ISCOM, immunology


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