TY - JOUR
T1 - Novel strategy for relieving acid accumulation by enriching syntrophic associations of syntrophic fatty acid-oxidation bacteria and H2/formate-scavenging methanogens in anaerobic digestion
AU - Lv, Nan
AU - Zhao, Lixin
AU - Wang, Ruming
AU - Ning, Jing
AU - Pan, Xiaofang
AU - Li, Chunxing
AU - Cai, Guanjing
AU - Zhu, Gefu
PY - 2020
Y1 - 2020
N2 - Aiming at relieving acid accumulation in anaerobic digestion (AD), syntrophic associations of syntrophic fatty acid-oxidation bacteria and H2/formate-scavenging methanogens were enriched by feeding propionate, butyrate and formate in an up-flow anaerobic sludge blanket (UASB) reactor. Results showed that methane yield increased by 50% with increasing formate concentration (0–2000 mg COD/L). In addition, the abundance and quantity of SFOB (Syntrophobacter, Smithella and Syntrophomonas) and H2/formate-scavenging methanogens (Methanobacteriales and Methanomicrobiales) were increased after microbial acclimation. The enriched syntrophic associations showed higher propionate and butyrate removal efficiencies of 98.48 ± 1.14% and 99.71 ± 0.71%, respectively. Furthermore, encoding genes of formate dehydrogenase and hydrogenases presented higher abundances after microbial enrichment, which suggested that the enhancements of interspecies formate transfer and interspecies hydrogen transfer between syntrophic associations benefited volatile fatty acids (VFAs) conversion. This research provided an effective strategy to relieve acid accumulation.
AB - Aiming at relieving acid accumulation in anaerobic digestion (AD), syntrophic associations of syntrophic fatty acid-oxidation bacteria and H2/formate-scavenging methanogens were enriched by feeding propionate, butyrate and formate in an up-flow anaerobic sludge blanket (UASB) reactor. Results showed that methane yield increased by 50% with increasing formate concentration (0–2000 mg COD/L). In addition, the abundance and quantity of SFOB (Syntrophobacter, Smithella and Syntrophomonas) and H2/formate-scavenging methanogens (Methanobacteriales and Methanomicrobiales) were increased after microbial acclimation. The enriched syntrophic associations showed higher propionate and butyrate removal efficiencies of 98.48 ± 1.14% and 99.71 ± 0.71%, respectively. Furthermore, encoding genes of formate dehydrogenase and hydrogenases presented higher abundances after microbial enrichment, which suggested that the enhancements of interspecies formate transfer and interspecies hydrogen transfer between syntrophic associations benefited volatile fatty acids (VFAs) conversion. This research provided an effective strategy to relieve acid accumulation.
KW - Acid accumulation
KW - Anaerobic digestion
KW - Formate
KW - Microbial enrichment
KW - Syntrophic associations
U2 - 10.1016/j.biortech.2020.123702
DO - 10.1016/j.biortech.2020.123702
M3 - Journal article
C2 - 32615503
AN - SCOPUS:85087025168
SN - 0960-8524
VL - 313
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 123702
ER -