Novel protocol for lutein extraction from microalga Chlorella vulgaris

Martina D'Este, Davide De Francisci, Irini Angelidaki

Research output: Contribution to journalJournal articleResearchpeer-review

300 Downloads (Pure)

Abstract

Lutein is a pigment generally extracted from marigold flowers. However, lutein is also found in considerable amounts in microalgae. In this study a novel method was developed to improve the extraction efficiency of lutein from microalga C. vulgaris. Differently from conventional methods, ethanol was used instead of water in the saponification step, which was conducted simultaneously to the solvent extraction, performed using dichloromethane. The amount of lutein extracted from C. vulgaris dried biomass increased more than threefold, from 0.20 ± 0.00 mgLutein/gDM to 0.69 ± 0.08 mgLutein/gDM. Lutein purity was increased from 73.6% to 93.7% by decreasing the ethanol-water ratio from 85% to 50% in the resolubilization step. The novel method was also tested with tetrahydrofuran. The extraction proved to be again more effective than the conventional one; however dichloromethane outperformed tetrahydrofuran in terms of quantity and purity of the recovered lutein.
Original languageEnglish
JournalBiochemical Engineering Journal
Volume127
Pages (from-to)175-179
ISSN1369-703X
DOIs
Publication statusPublished - 2017

Keywords

  • Lutein
  • Chlorella vulgaris
  • Extraction
  • saponification
  • Dichloromethane

Fingerprint Dive into the research topics of 'Novel protocol for lutein extraction from microalga Chlorella vulgaris'. Together they form a unique fingerprint.

Cite this