Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Mario Corral Bolaños; Tram Nguyen; Hans Martin Kjer; Marco Pizzolato; Casper Bo Gravesen Salinas; Johanna Perens; Jeppe Revall Frisvad; Julien Colombelli; Tim Bjørn Dyrby

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Mario Corral Bolaños, Tram Nguyen, Hans Martin Kjer, Marco Pizzolato, Casper Bo Gravesen Salinas, Johanna Perens, Jeppe Revall Frisvad, Julien Colombelli, Tim Bjørn Dyrby

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

Abstract

This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

Original language	English
Title of host publication	Proceedings of the ISMRM & ISMRT Annual Meeting & Exhibition 2023
Number of pages	2
Volume	31
Publisher	The International Society for Magnetic Resonance in Medicine
Publication date	2023
Article number	5013
Publication status	Published - 2023
Event	2023 ISMRM & ISMRT Annual Meeting & Exhibition - Metro Toronto Convention Centre (MTCC), Toronto, Canada Duration: 3 Jun 2023 → 8 Jun 2023 Conference number: 32

Conference

Conference	2023 ISMRM & ISMRT Annual Meeting & Exhibition
Number	32
Location	Metro Toronto Convention Centre (MTCC)
Country/Territory	Canada
City	Toronto
Period	03/06/2023 → 08/06/2023

Bibliographical note

Conference Abstract in proceedings - 2023, ISMRM 32nd Annual Meeting Proceedings. The International Society for Magnetic Resonance in Medicine

Keywords

Validation
Tractography & Fibre Modelling
3D-Fibre orientation

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Otto Mønsted Fond travel grant
Corral Bolaños, M. (Recipient), 2023
Prize: Prizes, scholarships, distinctions

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Corral Bolaños, M., Nguyen, T., Kjer, H. M., Pizzolato, M., Salinas, C. B. G., Perens, J., Frisvad, J. R., Colombelli, J., & Dyrby, T. B. (2023). Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter. In Proceedings of the ISMRM & ISMRT Annual Meeting & Exhibition 2023 (Vol. 31). Article 5013 The International Society for Magnetic Resonance in Medicine.

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title = "Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter",

abstract = "This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods. ",

keywords = "Validation, Tractography & Fibre Modelling, 3D-Fibre orientation",

author = "{Corral Bola{\~n}os}, Mario and Tram Nguyen and Kjer, {Hans Martin} and Marco Pizzolato and Salinas, {Casper Bo Gravesen} and Johanna Perens and Frisvad, {Jeppe Revall} and Julien Colombelli and Dyrby, {Tim Bj{\o}rn}",

note = "Conference Abstract in proceedings - 2023, ISMRM 32nd Annual Meeting Proceedings. The International Society for Magnetic Resonance in Medicine; 2023 ISMRM & ISMRT Annual Meeting & Exhibition ; Conference date: 03-06-2023 Through 08-06-2023",

year = "2023",

language = "English",

volume = "31",

booktitle = "Proceedings of the ISMRM & ISMRT Annual Meeting & Exhibition 2023",

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Corral Bolaños, M, Nguyen, T, Kjer, HM , Pizzolato, M, Salinas, CBG, Perens, J, Frisvad, JR, Colombelli, J & Dyrby, TB 2023, Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter. in Proceedings of the ISMRM & ISMRT Annual Meeting & Exhibition 2023. vol. 31, 5013, The International Society for Magnetic Resonance in Medicine, 2023 ISMRM & ISMRT Annual Meeting & Exhibition, Toronto, Ontario, Canada, 03/06/2023.

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter. / Corral Bolaños, Mario; Nguyen, Tram; Kjer, Hans Martin et al.
Proceedings of the ISMRM & ISMRT Annual Meeting & Exhibition 2023. Vol. 31 The International Society for Magnetic Resonance in Medicine, 2023. 5013.

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

TY - ABST

T1 - Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

AU - Corral Bolaños, Mario

AU - Nguyen, Tram

AU - Kjer, Hans Martin

AU - Pizzolato, Marco

AU - Salinas, Casper Bo Gravesen

AU - Perens, Johanna

AU - Frisvad, Jeppe Revall

AU - Colombelli, Julien

AU - Dyrby, Tim Bjørn

N1 - Conference code: 32

PY - 2023

Y1 - 2023

N2 - This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

AB - This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

KW - Validation

KW - Tractography & Fibre Modelling

KW - 3D-Fibre orientation

M3 - Conference abstract in proceedings

VL - 31

BT - Proceedings of the ISMRM & ISMRT Annual Meeting & Exhibition 2023

PB - The International Society for Magnetic Resonance in Medicine

T2 - 2023 ISMRM & ISMRT Annual Meeting & Exhibition

Y2 - 3 June 2023 through 8 June 2023

ER -

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Abstract

Conference

Bibliographical note

Keywords

OpenUrl availability

Embargoed Document

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Prizes

Otto Mønsted Fond travel grant

Cite this