Novel bioaugmentation strategy boosted with biochar to alleviate ammonia toxicity in continuous biomethanation

This study investigated for the first time if ammonia tolerant methanogenic consortia can be stored in gel (biogel) and used in a later time on-demand as bioaugmentation inocula, to efficiently relieve ammonia inhibition in continuous biomethanation systems. Moreover, wood biochar was assessed as a potential enhancer of the novel biogel bioaugmentation process. Three thermophilic (55 °C), continuous stirred-tank reactors (R_Bgel, R_Char and R_Mix), operated at 4.5 g NH₄⁺-N L^-1 were exposed to biogel, biochar and mixture of biogel and biochar, respectively, while a fourth reactor (R_Ctrl) was used as control. The results showed that the methane production yields of R_Mix, R_Char and R_Bgel increased by 28.6%, 20.2% and 10.7%, respectively compared to R_Ctrl. The highest methane yield was achieved by the synergistic interaction between biogel and biochar. Additionally, biogel stimulated a rapid recovery of Methanoculleus thermophilus sp. and syntrophic acetate oxidising bacteria populations.